| Literature DB >> 33511119 |
Felix Royo1,2, Mikel Azkargorta1, Jose L Lavin1, Marc Clos-Garcia1, Ana R Cortazar1,3, Monika Gonzalez-Lopez1, Laura Barcena1, Hernando A Del Portillo4,5,6, María Yáñez-Mó7, Antonio Marcilla8,9, Francesc E Borras10,11,12, Hector Peinado13, Isabel Guerrero14, Mar Váles-Gómez15, Unai Cereijo16, Teresa Sardon16, Ana M Aransay1,2, Felix Elortza1,2, Juan M Falcon-Perez1,2,17.
Abstract
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.Entities:
Keywords: MLP29; cell crosstalk; exosomes; extracellular vesicles (EVs); fibroblast; immune response
Year: 2021 PMID： 33511119 PMCID： PMC7835421 DOI： 10.3389/fcell.2020.613583
Source DB: PubMed Journal: Front Cell Dev Biol ISSN： 2296-634X