| Literature DB >> 33510099 |
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Abstract
[This corrects the article DOI: 10.4103/1673-5374.286977.Entities:
Year: 2021 PMID: 33510099 PMCID: PMC8328789 DOI: 10.4103/1673-5374.303538
Source DB: PubMed Journal: Neural Regen Res ISSN: 1673-5374 Impact factor: 5.135
Figure 2Preparation and detection of nerve microtissue.
(A) The cells proliferating from the microtissues were stained with S100 and DAPI, which confirmed that they were Schwann cells. The optical microscope images show enlarged views of the area in the black rectangle. With increasing time in culture, the proliferation of cells around the nerve microtissue increased gradually, and the morphology was still long and narrow. Scale bars: 500 µm in the microtissue column, 200 µm in the optical microscope column, 100 µm in the left two immunofluorescence columns, and 50 µm in the right immunofluorescence column. (B) The enzyme-linked immunosorbent assay results showed that nerve microtissues can secrete large amounts of NGF, VEGF and GDNF (n = 3 independently repeated assay for each group). Data are expressed as the mean ± SD. **P < 0.01 (one-way analysis of variance followed by Tukey's post hoc test). DAPI: 4',6-Diamidino2-phenylindole; GDNF: glial cell line-derived neurotrophic factor; NF200: neurofilament 200; NGF: nerve growth factor; n.s.: not significant; VEGF: vascular endothelial growth factor.