Soo Jin Jeon1, Federico Cunha2, Rodolfo Daetz2, Rodrigo C Bicalho3, Svetlana Lima3,4, Klibs N Galvão5,6. 1. Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, Brookville, NY, 11548, USA. soojin.jeon@liu.edu. 2. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA. 3. Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY, 14853, USA. 4. Present Address: Department of Medicine, Weill Cornell Medicine, New York, NY, 10065, USA. 5. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA. galvaok@ufl.edu. 6. D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL, 32611, USA. galvaok@ufl.edu.
Abstract
BACKGROUND: Metritis is an inflammatory uterine disease found in ~ 20% of dairy cows after parturition and associated with uterine microbiota with high abundance of Fusobacterium, Bacteroides, and Porphyromonas. Ceftiofur is a common treatment, but the effect on uterine microbiota is poorly understood. Herein, we investigated the short-term impact of ceftiofur on uterine microbiota structure and function in cows with metritis. Eight cows received ceftiofur (CEF) and 10 remained untreated (CON). Uterine swabs were collected for PCR and metagenomic analysis at diagnosis before treatment (5 ± 1 DPP) and 2 days after diagnosis/treatment (7 ± 1 DPP) from the same individuals. Seven CEF and 9 CON passed quality control and were used for 16S rRNA gene sequencing. RESULTS: Ceftiofur treatment resulted in uterine microbiota alteration, which was attributed to a decrease in relative abundance of Fusobacterium and in gene contents involved in lipopolysaccharide biosynthesis, whereas uterine microbiota diversity and genes involved in pantothenate and coenzyme A biosynthesis increased. Ceftiofur treatment also reduced rectal temperature and tended to reduce total bacteria in the uterus. However, other uterine pathogens such as Bacteroides and Porphyromonas remained unchanged in CEF. The blaCTX-M gene was detected in 37.5% of metritic cows tested but was not affected by CEF. We found that β-hydroxybutyric acid, pyruvic acid, and L-glutamine were preferentially utilized by Fusobacterium necrophorum according to metabolic activity with 95 carbon sources. CONCLUSIONS: Ceftiofur treatment leads to alterations in the uterine microbiota that were mainly characterized by reductions in Fusobacterium and genes involved in LPS biosynthesis, which may be associated with a decrease in rectal temperature. The increase in pantothenate and coenzyme A biosynthesis indicates microbial response to metabolic stress caused by ceftiofur. Preference of Fusobacterium for β-hydroxybutyric acid may help to explain why this strain becomes dominant in the uterine microbiota of cows with metritis, and it also may provide a means for development of new therapies for the control of metritis in dairy cows.
BACKGROUND: Metritis is an inflammatory uterine disease found in ~ 20% of dairy cows after parturition and associated with uterine microbiota with high abundance of Fusobacterium, Bacteroides, and Porphyromonas. Ceftiofur is a common treatment, but the effect on uterine microbiota is poorly understood. Herein, we investigated the short-term impact of ceftiofur on uterine microbiota structure and function in cows with metritis. Eight cows received ceftiofur (CEF) and 10 remained untreated (CON). Uterine swabs were collected for PCR and metagenomic analysis at diagnosis before treatment (5 ± 1 DPP) and 2 days after diagnosis/treatment (7 ± 1 DPP) from the same individuals. Seven CEF and 9 CON passed quality control and were used for 16S rRNA gene sequencing. RESULTS:Ceftiofur treatment resulted in uterine microbiota alteration, which was attributed to a decrease in relative abundance of Fusobacterium and in gene contents involved in lipopolysaccharide biosynthesis, whereas uterine microbiota diversity and genes involved in pantothenate and coenzyme A biosynthesis increased. Ceftiofur treatment also reduced rectal temperature and tended to reduce total bacteria in the uterus. However, other uterine pathogens such as Bacteroides and Porphyromonas remained unchanged in CEF. The blaCTX-M gene was detected in 37.5% of metritic cows tested but was not affected by CEF. We found that β-hydroxybutyric acid, pyruvic acid, and L-glutamine were preferentially utilized by Fusobacterium necrophorum according to metabolic activity with 95 carbon sources. CONCLUSIONS:Ceftiofur treatment leads to alterations in the uterine microbiota that were mainly characterized by reductions in Fusobacterium and genes involved in LPS biosynthesis, which may be associated with a decrease in rectal temperature. The increase in pantothenate and coenzyme A biosynthesis indicates microbial response to metabolic stress caused by ceftiofur. Preference of Fusobacterium for β-hydroxybutyric acid may help to explain why this strain becomes dominant in the uterine microbiota of cows with metritis, and it also may provide a means for development of new therapies for the control of metritis in dairy cows.
Authors: Soo Jin Jeon; Fabio S Lima; Achilles Vieira-Neto; Vinicius S Machado; Svetlana F Lima; Rodrigo C Bicalho; Jose Eduardo P Santos; Klibs N Galvão Journal: Vet Microbiol Date: 2017-12-28 Impact factor: 3.293
Authors: F S Lima; A Vieira-Neto; G S F M Vasconcellos; R D Mingoti; E Karakaya; E Solé; R S Bisinotto; N Martinez; C A Risco; K N Galvão; J E P Santos Journal: J Dairy Sci Date: 2014-06-18 Impact factor: 4.034
Authors: N Martinez; C A Risco; F S Lima; R S Bisinotto; L F Greco; E S Ribeiro; F Maunsell; K Galvão; J E P Santos Journal: J Dairy Sci Date: 2012-09-26 Impact factor: 4.034
Authors: Soo Jin Jeon; Achilles Vieira-Neto; Mohanathas Gobikrushanth; Rodolfo Daetz; Rodolfo D Mingoti; Ana Carolina Brigolin Parize; Sabrina Lucas de Freitas; Antonio Nelson Lima da Costa; Rodrigo C Bicalho; Svetlana Lima; K Casey Jeong; Klibs N Galvão Journal: Appl Environ Microbiol Date: 2015-07-06 Impact factor: 4.792
Authors: Soo Jin Jeon; Federico Cunha; Xiaojie Ma; Natalia Martinez; Achilles Vieira-Neto; Rodolfo Daetz; Rodrigo C Bicalho; Svetlana Lima; Jose E P Santos; K Casey Jeong; Klibs N Galvão Journal: PLoS One Date: 2016-11-01 Impact factor: 3.240
Authors: Ron Sicsic; Tamir Goshen; Rahul Dutta; Noa Kedem-Vaanunu; Veronica Kaplan-Shabtai; Zohar Pasternak; Yuval Gottlieb; Nahum Y Shpigel; Tal Raz Journal: Vet Res Date: 2018-08-02 Impact factor: 3.683
Authors: Samat Amat; Carl R Dahlen; Kendall C Swanson; Alison K Ward; Lawrence P Reynolds; Joel S Caton Journal: Front Microbiol Date: 2022-02-23 Impact factor: 5.640