Zhongbao Wu1. 1. Department of Neurosurgery, The Third People's Hospital of Datong City, Datong, P.R. China.
Abstract
AIMS: LncRNA RUNX1-IT1 has been characterized as a tumor suppressive lncRNA in several cancers, while its role in glioblastoma (GBM) is unknown. This study aimed to investigate the potential involvement of RUNX1-IT1 in GBM. METHODS: Expression of RUNX1-IT1 in GBM tissues and paired non-tumor tissues was determined by RT-qPCR. The interaction between RUNX1-IT1 and miR-195 was analyzed by dual luciferase activity assay. Overexpression of RUNX1-IT1 and miR-195 was achieved in GBM cells to explore the interaction between them. The effects of RUNX1-IT1 and miR-195 overexpression on the expression of cyclin D1 were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay. RESULTS: RUNX1-IT1 was upregulated in GBM. RUNX1-IT1 and miR-195 interacted with each other, but failed to regulate the expression of each other. Overexpression of RUNX1-IT1 resulted in the upregulation of cyclin D1, and also reduced the effects of miR-195 overexpression on cyclin D1 expression. RUNX1-IT1 and cyclin overexpression increased cell proliferation, while miR-195 overexpression decreased cell proliferation. In addition, RUNX1-IT1 overexpression reduced the effects of miR-195 overexpression on cell proliferation. CONCLUSIONS: RUNX1-IT1 may sponge miR-195 to upregulate cyclin D1, thereby increasing the proliferation of glioblastoma cells.
AIMS: LncRNA RUNX1-IT1 has been characterized as a tumor suppressive lncRNA in several cancers, while its role in glioblastoma (GBM) is unknown. This study aimed to investigate the potential involvement of RUNX1-IT1 in GBM. METHODS: Expression of RUNX1-IT1 in GBM tissues and paired non-tumor tissues was determined by RT-qPCR. The interaction between RUNX1-IT1 and miR-195 was analyzed by dual luciferase activity assay. Overexpression of RUNX1-IT1 and miR-195 was achieved in GBM cells to explore the interaction between them. The effects of RUNX1-IT1 and miR-195 overexpression on the expression of cyclin D1 were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay. RESULTS: RUNX1-IT1 was upregulated in GBM. RUNX1-IT1 and miR-195 interacted with each other, but failed to regulate the expression of each other. Overexpression of RUNX1-IT1 resulted in the upregulation of cyclin D1, and also reduced the effects of miR-195 overexpression on cyclin D1 expression. RUNX1-IT1 and cyclin overexpression increased cell proliferation, while miR-195 overexpression decreased cell proliferation. In addition, RUNX1-IT1 overexpression reduced the effects of miR-195 overexpression on cell proliferation. CONCLUSIONS: RUNX1-IT1 may sponge miR-195 to upregulate cyclin D1, thereby increasing the proliferation of glioblastoma cells.
Authors: Yu Zhang; Kun Zhuang; Shanshan Yuan; Wangli Si; Yijun Li; Jun Zhang; Jiaming Liu Journal: Comput Math Methods Med Date: 2022-07-26 Impact factor: 2.809