Satish Datla1, Srinivasan Kitchanan1, Giridhar Sethuraman2. 1. Department of Neonatology, Chettinad Hospital and Research Institute, Chettinad Health City, Kelambakkam, Chengalpattu, Tamil Nadu, India. 2. Department of Neonatology, Chettinad Hospital and Research Institute, Chettinad Health City, Kelambakkam, Chengalpattu, Tamil Nadu, India. Correspondence to: Dr Giridhar Sethuraman, Department of Neonatology, Chettinad Hospital and Research Institute, Chettinad Health City, Rajiv Gandhi IT highway, Kelambakkam, Chengalpattu, Tamil Nadu 603 103. giridharsethu@gmail.com.
Abstract
OBJECTIVE: To assess if salivary C-reactive protein (CRP) can be detected in neonatal sepsis and correlate the levels of salivary and serum CRP. METHODS: This analytical cross-sectional study included all neonates £28 days of life with suspected sepsis or with perinatal risk factors for sepsis. Saliva was collected using an absorbent swab and analyzed by enzyme-linked immunosorbent assay, along with serum CRP. RESULTS: Salivary CRP was detectable in 135 subjects (99%). An increase was seen in median (IQR) levels from 0.25 (0.13,0.3) ng/mL in clinical sepsis group to 0.6 (0.3,1.4) ng/mL in screen positive/blood culture negative group, and to 1.98 (0.54, 2.95) ng/mL in blood culture positive group. There was a moderate positive correlation between salivary and serum CRP (r=0.63, P value 0.01). On receiver-operator characteristics curve, the area under the curve of salivary CRP for predicting serum CRP ≥10 mg/L was 0.861 (95% CI, 0.78 to 0.94; P <0.001), with the optimal salivary CRP cut-off being 0.6 ng/mL. CONCLUSION: Salivary CRP could be used as an alternative biomarker of neonatal sepsis.
OBJECTIVE: To assess if salivary C-reactive protein (CRP) can be detected in neonatal sepsis and correlate the levels of salivary and serum CRP. METHODS: This analytical cross-sectional study included all neonates £28 days of life with suspected sepsis or with perinatal risk factors for sepsis. Saliva was collected using an absorbent swab and analyzed by enzyme-linked immunosorbent assay, along with serum CRP. RESULTS: Salivary CRP was detectable in 135 subjects (99%). An increase was seen in median (IQR) levels from 0.25 (0.13,0.3) ng/mL in clinical sepsis group to 0.6 (0.3,1.4) ng/mL in screen positive/blood culture negative group, and to 1.98 (0.54, 2.95) ng/mL in blood culture positive group. There was a moderate positive correlation between salivary and serum CRP (r=0.63, P value 0.01). On receiver-operator characteristics curve, the area under the curve of salivary CRP for predicting serum CRP ≥10 mg/L was 0.861 (95% CI, 0.78 to 0.94; P <0.001), with the optimal salivary CRP cut-off being 0.6 ng/mL. CONCLUSION: Salivary CRP could be used as an alternative biomarker of neonatal sepsis.
Authors: Sumeet Manandhar; Amy Scott-Thomas; Michael Harrington; Priyanka Sinha; Anna Pilbrow; Arthur Mark Richards; Vicky Cameron; Madhav Bhatia; Stephen T Chambers Journal: Int J Mol Sci Date: 2022-08-03 Impact factor: 6.208