Wenyu Wang1, Jing Wang2, Hong Chen1, Xiaofei Zhang1, Kaiyu Han1. 1. Department of Respiratory and Critical Medicine, The Second Affiliated Hospital of Harbin Medical University, China. 2. Department of Respiratory Medicine, Harbin Chest Hospital, China.
Abstract
BACKGROUND: The present study focused on the potential clinical significance of miR-3934 in the occurrence and development of asthma. METHODS: 80 asthma and 80 healthy controls were recruited in this study. The peripheral blood mononuclear cells (PBMCs) and serum samples of the asthma patients as well as the healthy controls were isolated, and the expression levels of miR-3934 in PBMCs were examined by RT-qPCR methods. Furthermore, the relationship between the level of miR-3934 in PBMCs and the disease severity has been analyzed, and the potential diagnostic value of miR-3934 was evaluated by the receiver operating characteristics (ROC) curve. Finally, the expression level of IL-6, IL-8, and IL-33 have been detected using the ELISA kits, and Pearson's correlation analysis was performed to investigate the relationship between the level of miR-3934 in PBMCs and the serum expression of those inflammatory cytokines in asthma patients. RESULTS: miR-3934 was dramatically decreased in PBMCs of the asthma patients, and miR-3934 was markedly reduced in PBMCs of patients with severe asthma vs. mild asthma. Furthermore, ROC analysis showed that levels of miR-3934 in PBMCs can distinguish asthma patient, especially the severe asthma patients from the controls. Finally, the levels of miR-3934 in PBMCs were negatively correlated with the serum levels of IL-6, IL-8, and IL-33 in asthma patients, respectively. CONCLUSIONS: miR-3934 was downregulated in PBMCs of asthmatic patients and may function as a potential diagnosis biomarker.
BACKGROUND: The present study focused on the potential clinical significance of miR-3934 in the occurrence and development of asthma. METHODS: 80 asthma and 80 healthy controls were recruited in this study. The peripheral blood mononuclear cells (PBMCs) and serum samples of the asthma patients as well as the healthy controls were isolated, and the expression levels of miR-3934 in PBMCs were examined by RT-qPCR methods. Furthermore, the relationship between the level of miR-3934 in PBMCs and the disease severity has been analyzed, and the potential diagnostic value of miR-3934 was evaluated by the receiver operating characteristics (ROC) curve. Finally, the expression level of IL-6, IL-8, and IL-33 have been detected using the ELISA kits, and Pearson's correlation analysis was performed to investigate the relationship between the level of miR-3934 in PBMCs and the serum expression of those inflammatory cytokines in asthma patients. RESULTS: miR-3934 was dramatically decreased in PBMCs of the asthma patients, and miR-3934 was markedly reduced in PBMCs of patients with severe asthma vs. mild asthma. Furthermore, ROC analysis showed that levels of miR-3934 in PBMCs can distinguish asthma patient, especially the severe asthma patients from the controls. Finally, the levels of miR-3934 in PBMCs were negatively correlated with the serum levels of IL-6, IL-8, and IL-33 in asthma patients, respectively. CONCLUSIONS: miR-3934 was downregulated in PBMCs of asthmatic patients and may function as a potential diagnosis biomarker.
Authors: Timothy N Perkins; Elizabeth A Oczypok; Regina E Dutz; Mason L Donnell; Michael M Myerburg; Tim D Oury Journal: J Allergy Clin Immunol Date: 2019-03-30 Impact factor: 10.793