Mahvash Alizade Naini1,2, Shayan Mehrvarzi3, Asal Zargari-Samadnejadi3, Nader Tanideh4, Mohammad Ghorbani5, Amirreza Dehghanian6,7, Maryam Hasanzarrini8, Farnaz Banaee9, Omid Koohi-Hosseinabadi10,11, Cambyz Irajie12, Aida Iraji10,13. 1. Gastroenterhepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Health Sciences Research Center, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. 6. Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 7. Molecular Pathology and Cytogenetics Division, Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. 8. Department of Internal Medicine, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran. 9. Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 10. Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran. 11. Laparoscopy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 12. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. 13. Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
OBJECTIVES: Ulcerative colitis is a common subtype of persistent inflammatory bowel disease with high morbidity consequences. Despite unknown definite pathogenesis, multiple anti-inflammatory medications are used for its treatment. Traditionally, Quercus brantii (QB), mostly available in the Middle East, has been used for gastrointestinal disorders. Other beneficial effects associated with QB include reduction of oxidative stress, inflammations, homeostatic instability, and improvement in clinical conditions. MATERIALS AND METHODS: This experimental study was designed to assess the possible therapeutic effects of QB on UC and compare its effects with those of sulfasalazine. Of the 70 Wistar rats clustered in seven groups, ten received only alcohols and sixty were confirmed to be suffering from trinitrobenzene sulfonic acid- (TNBS-) induced colitis. Four groups received different dosages of QB extract via oral and rectal routes, one received sulfasalazine, and the other remaining two groups received nothing. The effects of QB were evaluated by assessing macroscopic and histologic scoring, measuring inflammatory mediators, and determining oxidative stress markers. RESULTS: Comparing to the untreated TNBS-induced control groups, QB-treated groups showed a dose- and route-dependent improvement comparable with sulfasalazine. Treating rats with QB reduced the microscopic and macroscopic damage, decreased TNF-α, IL-6, NO, MPO activity, and MDA content, increased superoxide dismutase (SOD) activity, and reduced body weight loss. CONCLUSIONS: Our data recommended the anti-inflammatory and antioxidant effects of QB extract in a dose-dependent manner.
OBJECTIVES: Ulcerative colitis is a common subtype of persistent inflammatory bowel disease with high morbidity consequences. Despite unknown definite pathogenesis, multiple anti-inflammatory medications are used for its treatment. Traditionally, Quercus brantii (QB), mostly available in the Middle East, has been used for gastrointestinal disorders. Other beneficial effects associated with QB include reduction of oxidative stress, inflammations, homeostatic instability, and improvement in clinical conditions. MATERIALS AND METHODS: This experimental study was designed to assess the possible therapeutic effects of QB on UC and compare its effects with those of sulfasalazine. Of the 70 Wistar rats clustered in seven groups, ten received only alcohols and sixty were confirmed to be suffering from trinitrobenzene sulfonic acid- (TNBS-) induced colitis. Four groups received different dosages of QB extract via oral and rectal routes, one received sulfasalazine, and the other remaining two groups received nothing. The effects of QB were evaluated by assessing macroscopic and histologic scoring, measuring inflammatory mediators, and determining oxidative stress markers. RESULTS: Comparing to the untreated TNBS-induced control groups, QB-treated groups showed a dose- and route-dependent improvement comparable with sulfasalazine. Treating rats with QB reduced the microscopic and macroscopic damage, decreased TNF-α, IL-6, NO, MPO activity, and MDA content, increased superoxide dismutase (SOD) activity, and reduced body weight loss. CONCLUSIONS: Our data recommended the anti-inflammatory and antioxidant effects of QB extract in a dose-dependent manner.