Micro-microbial electrochemical sensor equipped with combined bioanode and biocathode for water biotoxicity monitoring.

The development of low-cost biosensors for water monitoring is expected to reduce potential risks from contamination accidents. This study reported a novel micro-microbial electrochemical sensor using combined bioanode and biocathode as the sensing element, characterized by a sequential flowing membrane-free channel and a bilateral passive oxygen supply. A decrease in the ratio of number of bioanode to biocathode resulted in a lower power generation, whereas, achieving a similar or even higher toxic response. The voltage was affected by both the flow rate and the acetate concentration. With the increased acetate concentration, a clear trade-off was observed between the electroactivity stimulation of bioanode vs. the electroactivity maintenance of biocathode. Biosensors made good response to the injection of formaldehyde (10 µL of 0.25%, and 100 µL of 0.025%) into the inlet. A high microbial diversity was observed. This work can lead to a revolutionizing way of water monitoring using self-powered micro-biosensors.