Emily T Dodd1, Melissa L Pierce2, Jonathan S F Lee3, Rachel S Poretsky1. 1. Department of Biological Sciences, University of Illinois at Chicago, 845 W. Taylor Street, Chicago, IL, 60607, USA. 2. Department of Biological Sciences, University of Illinois at Chicago, 845 W. Taylor Street, Chicago, IL, 60607, USA. mlp16@uic.edu. 3. Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 7305 Beach Drive E, Port Orchard, WA, 98366, USA.
Abstract
BACKGROUND: The skin microbiome of marine fish is thought to come from bacteria in the surrounding water during the larval stages, although it is not clear how different water conditions affect the microbial communities in the water and, in turn, the composition and development of the larval skin microbiome. In aquaculture, water conditions are especially important; claywater and greenwater are often used in larval rearing tanks to increase water turbidity. Here, we explored the effects of these water additives on microbial communities in rearing water and on the skin of first-feeding sablefish larvae using 16S rRNA gene sequencing. We evaluated three treatments: greenwater, claywater, and greenwater with a switch to claywater after 1 week. RESULTS: We observed additive-specific effects on rearing water microbial communities that coincided with the addition of larvae and rotifer feed to the tanks, such as an increase in Vibrionaceae in greenwater tanks. Additionally, microbial communities from experimental tank water, especially those in claywater, began to resemble larval skin microbiomes by the end of the experiment. The differential effects of the additives on larval sablefish skin microbiomes were largest during the first week, post-first feed. Bacteria associated with greenwater, including Vibrionaceae and Pseudoalteromonas spp., were found on larval skin a week after the switch to claywater. In addition to additive-specific effects, larval skin microbiomes also retained bacterial families likely acquired from their hatchery silos. CONCLUSIONS: Our results suggest that larval sablefish skin microbiomes are most sensitive to the surrounding seawater up to 1 week following the yolk-sac stage and that claywater substituted for greenwater after 1 week post-first feed does not significantly impact skin-associated microbial communities. However, the larval skin microbiome changes over time under all experimental conditions. Furthermore, our findings suggest a potential two-way interaction between microbial communities on the host and the surrounding environment. To our knowledge, this is one of the few studies to suggest that fish might influence the microbial community of the seawater.
BACKGROUND: The skin microbiome of marine fish is thought to come from bacteria in the surrounding water during the larval stages, although it is not clear how different water conditions affect the microbial communities in the water and, in turn, the composition and development of the larval skin microbiome. In aquaculture, water conditions are especially important; claywater and greenwater are often used in larval rearing tanks to increase water turbidity. Here, we explored the effects of these water additives on microbial communities in rearing water and on the skin of first-feeding sablefish larvae using 16S rRNA gene sequencing. We evaluated three treatments: greenwater, claywater, and greenwater with a switch to claywater after 1 week. RESULTS: We observed additive-specific effects on rearing water microbial communities that coincided with the addition of larvae and rotifer feed to the tanks, such as an increase in Vibrionaceae in greenwater tanks. Additionally, microbial communities from experimental tank water, especially those in claywater, began to resemble larval skin microbiomes by the end of the experiment. The differential effects of the additives on larval sablefish skin microbiomes were largest during the first week, post-first feed. Bacteria associated with greenwater, including Vibrionaceae and Pseudoalteromonas spp., were found on larval skin a week after the switch to claywater. In addition to additive-specific effects, larval skin microbiomes also retained bacterial families likely acquired from their hatchery silos. CONCLUSIONS: Our results suggest that larval sablefish skin microbiomes are most sensitive to the surrounding seawater up to 1 week following the yolk-sac stage and that claywater substituted for greenwater after 1 week post-first feed does not significantly impact skin-associated microbial communities. However, the larval skin microbiome changes over time under all experimental conditions. Furthermore, our findings suggest a potential two-way interaction between microbial communities on the host and the surrounding environment. To our knowledge, this is one of the few studies to suggest that fish might influence the microbial community of the seawater.
Authors: Michael P Doane; John Matthew Haggerty; Dovi Kacev; Bhavya Papudeshi; Elizabeth A Dinsdale Journal: Environ Microbiol Rep Date: 2017-06-27 Impact factor: 3.541
Authors: Dan Knights; Justin Kuczynski; Emily S Charlson; Jesse Zaneveld; Michael C Mozer; Ronald G Collman; Frederic D Bushman; Rob Knight; Scott T Kelley Journal: Nat Methods Date: 2011-07-17 Impact factor: 28.547
Authors: Amy Apprill; Jooke Robbins; A Murat Eren; Adam A Pack; Julie Reveillaud; David Mattila; Michael Moore; Misty Niemeyer; Kathleen M T Moore; Tracy J Mincer Journal: PLoS One Date: 2014-03-26 Impact factor: 3.240
Authors: Molly C Bletz; Holly Archer; Reid N Harris; Valerie J McKenzie; Falitiana C E Rabemananjara; Andolalao Rakotoarison; Miguel Vences Journal: Front Microbiol Date: 2017-08-17 Impact factor: 5.640