Masataka Aoki1, Yukina Miyashita2, P Thao Tran3, Yoshiharu Okuno4, Takahiro Watari5, Takashi Yamaguchi3,5. 1. Department of Civil Engineering, National Institute of Technology, Wakayama College, 77 Noshima, Nada, Gobo, Wakayama, 644-0023, Japan. aoki@wakayama-nct.ac.jp. 2. Department of Civil Engineering, National Institute of Technology, Wakayama College, 77 Noshima, Nada, Gobo, Wakayama, 644-0023, Japan. 3. Department of Science of Technology Innovation, Nagaoka University of Technology, Nagaoka, Niigata, Japan. 4. Department of Applied Chemistry and Biochemistry, National Institute of Technology, Wakayama College, Gobo, Wakayama, Japan. 5. Department of Civil and Environmental Engineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan.
Abstract
OBJECTIVE: Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms. RESULTS: We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h-1. 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria. CONCLUSIONS: Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.
OBJECTIVE: Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms. RESULTS: We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h-1. 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria. CONCLUSIONS: Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.
Authors: José M Cerrato; Joseph O Falkinham; Andrea M Dietrich; William R Knocke; Chad W McKinney; Amy Pruden Journal: Water Res Date: 2010-05-04 Impact factor: 11.236
Authors: J Gregory Caporaso; Christian L Lauber; William A Walters; Donna Berg-Lyons; James Huntley; Noah Fierer; Sarah M Owens; Jason Betley; Louise Fraser; Markus Bauer; Niall Gormley; Jack A Gilbert; Geoff Smith; Rob Knight Journal: ISME J Date: 2012-03-08 Impact factor: 10.302
Authors: Tsing Bohu; Cara M Santelli; Denise M Akob; Thomas R Neu; Valerian Ciobota; Petra Rösch; Jürgen Popp; Sándor Nietzsche; Kirsten Küsel Journal: Front Microbiol Date: 2015-07-17 Impact factor: 5.640