Grzegorz Szewczyk1,2, Michał Pyzlak3, Katarzyna Pankiewicz4, Ewa Szczerba5, Aleksandra Stangret6, Dariusz Szukiewicz6, Marta Skoda6, Joanna Bierła7, Bożena Cukrowska7, Anna Fijałkowska5. 1. Department of General and Experimental Pathology, Medical University of Warsaw, Warsaw, Poland. grzegorz.szewczyk@wum.edu.pl. 2. Department of Obstetrics and Gynecology, Institute of Mother and Child, Warsaw, Poland. grzegorz.szewczyk@wum.edu.pl. 3. Department of Pathology, Maria Sklodowska-Curie Institute-Oncology Center, Warsaw, Poland. 4. Department of Obstetrics and Gynecology, Institute of Mother and Child, Warsaw, Poland. 5. Department of Cardiology, Institute of Mother and Child, Warsaw, Poland. 6. Department of General and Experimental Pathology, Medical University of Warsaw, Warsaw, Poland. 7. Department of Pathology, Children's Memorial Health Institute, Warsaw, Poland.
Abstract
PURPOSE: Impaired angiogenesis is one of the most common findings in preeclamptic placentas. A new angiogenetic role of fractalkine (CX3CL1) is recently recognized apart from inflammatory activity. In this study, a link between CX3CL1 and the development of placental vasculature in preeclampsia was examined. METHODS: The study comprised 52 women allocated to Group 1 (normotensive, n = 23) and Group 2 (preeclampsia, n = 29). In each group Doppler parameters, serum levels of CX3CL1, soluble fms-like tyrosine kinase-1 (sFlt-1), and placental growth factor (PlGF) were assessed between 30 and 32 week of pregnancy. After the delivery, placental samples were taken and the vascularization and expression of CX3CR1 receptor were assessed after immunostaining. RESULTS: CX3CL1 and sFlt-1 serum levels were significantly higher levels in Group 2 vs Group 1, while PlGF serum levels was significantly lower in Group 2. Lower cerebroplacental ratio (CPR) was observed in Group 2. The vascular/extravascular tissue index (V/EVTI) was significantly lower in Group 2, while compared to Group 1, with the lowest value in the fetus growth restriction (FGR) subgroup (0.18 ± 0.02; 0.24 ± 0.03; 0.16 ± 0.02, respectively). The expression of examined CX3CR1 was higher in Group 2, while compared to Group 1, reaching the highest values in FGR subgroup. There was a moderate negative correlation between birth weight, V/EVTI and CX3CL1 serum level and CX3CR1 placental expression in the group of pregnancies complicated with preeclampsia. CONCLUSION: The significant underdevelopment of placental vascular network in preeclampsia is associated with the change in the CX3CL1/CX3CR1 system, especially in FGR complicated pregnancies.
PURPOSE: Impaired angiogenesis is one of the most common findings in preeclamptic placentas. A new angiogenetic role of fractalkine (CX3CL1) is recently recognized apart from inflammatory activity. In this study, a link between CX3CL1 and the development of placental vasculature in preeclampsia was examined. METHODS: The study comprised 52 women allocated to Group 1 (normotensive, n = 23) and Group 2 (preeclampsia, n = 29). In each group Doppler parameters, serum levels of CX3CL1, soluble fms-like tyrosine kinase-1 (sFlt-1), and placental growth factor (PlGF) were assessed between 30 and 32 week of pregnancy. After the delivery, placental samples were taken and the vascularization and expression of CX3CR1 receptor were assessed after immunostaining. RESULTS:CX3CL1 and sFlt-1 serum levels were significantly higher levels in Group 2 vs Group 1, while PlGF serum levels was significantly lower in Group 2. Lower cerebroplacental ratio (CPR) was observed in Group 2. The vascular/extravascular tissue index (V/EVTI) was significantly lower in Group 2, while compared to Group 1, with the lowest value in the fetus growth restriction (FGR) subgroup (0.18 ± 0.02; 0.24 ± 0.03; 0.16 ± 0.02, respectively). The expression of examined CX3CR1 was higher in Group 2, while compared to Group 1, reaching the highest values in FGR subgroup. There was a moderate negative correlation between birth weight, V/EVTI and CX3CL1 serum level and CX3CR1 placental expression in the group of pregnancies complicated with preeclampsia. CONCLUSION: The significant underdevelopment of placental vascular network in preeclampsia is associated with the change in the CX3CL1/CX3CR1 system, especially in FGR complicated pregnancies.
Authors: Karen D Cowden Dahl; Benjamin H Fryer; Fiona A Mack; Veerle Compernolle; Emin Maltepe; David M Adelman; Peter Carmeliet; M Celeste Simon Journal: Mol Cell Biol Date: 2005-12 Impact factor: 4.272
Authors: Miguel A Ortega; Ana M Gómez-Lahoz; Lara Sánchez-Trujillo; Oscar Fraile-Martinez; Cielo García-Montero; Luis G Guijarro; Coral Bravo; Juan A De Leon-Luis; Jose V Saz; Julia Bujan; Natalio García-Honduvilla; Jorge Monserrat; Melchor Alvarez-Mon Journal: Int J Mol Sci Date: 2022-08-11 Impact factor: 6.208