Qi Wu1,2, Zhixian Lin1,2, Jinsen Wu1,2, Kun Qian1,2,3, Hongxia Shao1,2,3, Jianqiang Ye4,5,6,7,8, Aijian Qin9,10,11,12,13. 1. Key Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. 2. Jiangsu Key Lab of Zoonosis, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. 3. The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. 4. Key Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. jqye@yzu.edu.cn. 5. Jiangsu Key Lab of Zoonosis, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. jqye@yzu.edu.cn. 6. The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. jqye@yzu.edu.cn. 7. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. jqye@yzu.edu.cn. 8. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. jqye@yzu.edu.cn. 9. Key Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. aijian@yzu.edu.cn. 10. Jiangsu Key Lab of Zoonosis, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. aijian@yzu.edu.cn. 11. The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. aijian@yzu.edu.cn. 12. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. aijian@yzu.edu.cn. 13. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, 225009, Yangzhou, Jiangsu, P.R. China. aijian@yzu.edu.cn.
Abstract
BACKGROUND: Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies. RESULTS: In this study, a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine was developed. The pELISA could indirect evaluate neutralizing antibody titers against different types of IBV in all tested sera. The titers measured with the pELISA had a coefficient of 0.83 for neutralizing antibody titers. CONCLUSIONS: The pELISA could detect antibodies against different types of IBV in all tested sera. The pELISA has the potential to evaluate samples for IBV-specific neutralizing antibodies and surveillance the infection of IBV.
BACKGROUND:Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies. RESULTS: In this study, a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine was developed. The pELISA could indirect evaluate neutralizing antibody titers against different types of IBV in all tested sera. The titers measured with the pELISA had a coefficient of 0.83 for neutralizing antibody titers. CONCLUSIONS: The pELISA could detect antibodies against different types of IBV in all tested sera. The pELISA has the potential to evaluate samples for IBV-specific neutralizing antibodies and surveillance the infection of IBV.
Authors: Aliandra M Gibertoni; Maria de Fátima S Montassier; Janete A D Sena; Patrícia E N Givisiez; Cibele R A G Furuyama; Hélio J Montassier Journal: J Clin Microbiol Date: 2005-04 Impact factor: 5.948
Authors: Alexandra C Walls; Young-Jun Park; M Alejandra Tortorici; Abigail Wall; Andrew T McGuire; David Veesler Journal: Cell Date: 2020-03-09 Impact factor: 41.582