María E Sánchez-Sandoval1, Victor M González-Mendoza2, Graciela E Racagni Di-Palma3, Yahaira A Cab-Guillén1, José A Muñoz-Sanchez1, Ana Ramos-Díaz4, S M Teresa Hernández-Sotomayor5. 1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico. 2. CONA CYT- Centro de Investigación y Desarrollo en Agrobiotecnología Alimentaria (Consortium between Centro de Investigación y Desarrollo, A.C. and Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco), San Agustín Tlaxiaca, Hidalgo, Mexico. 3. Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. 4. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Subsede Sureste, Yucatán, Mexico. 5. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico. ths@cicy.mx.
Abstract
BACKGROUND: Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general. Studies have been carried out with isolated strains of Colletotrichum in Capsicum plants; however, under growing conditions, microorganisms generally interact with others, resulting in an increase or decrease of their ability to infect the roots of C. chinense seedlings and thus, cause disease. RESULTS: Morphological changes were evident 24 h after inoculation (hai) with the microbial consortium, which consisted primarily of C. ignotum. High levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found around 6 hai. These metabolic changes could be correlated with high transcription levels of diacylglycerol-kinase (CchDGK1 and CchDG31) at 3, 6 and 12 hai and also to pathogen gene markers, such as CchPR1 and CchPR5. CONCLUSIONS: Our data constitute the first evidence for the phospholipids signalling events, specifically DGPP and PA participation in the phospholipase C/DGK (PI-PLC/DGK) pathway, in the response of Capsicum to the consortium, offering new insights on chilis' defense responses to damping-off diseases.
BACKGROUND: Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general. Studies have been carried out with isolated strains of Colletotrichum in Capsicum plants; however, under growing conditions, microorganisms generally interact with others, resulting in an increase or decrease of their ability to infect the roots of C. chinense seedlings and thus, cause disease. RESULTS: Morphological changes were evident 24 h after inoculation (hai) with the microbial consortium, which consisted primarily of C. ignotum. High levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found around 6 hai. These metabolic changes could be correlated with high transcription levels of diacylglycerol-kinase (CchDGK1 and CchDG31) at 3, 6 and 12 hai and also to pathogen gene markers, such as CchPR1 and CchPR5. CONCLUSIONS: Our data constitute the first evidence for the phospholipids signalling events, specifically DGPP and PA participation in the phospholipase C/DGK (PI-PLC/DGK) pathway, in the response of Capsicum to the consortium, offering new insights on chilis' defense responses to damping-off diseases.
Authors: F Meyer; D Paarmann; M D'Souza; R Olson; E M Glass; M Kubal; T Paczian; A Rodriguez; R Stevens; A Wilke; J Wilkening; R A Edwards Journal: BMC Bioinformatics Date: 2008-09-19 Impact factor: 3.169
Authors: Yi Zhou; David R Coventry; Vadakattu V S R Gupta; David Fuentes; Andrew Merchant; Brent N Kaiser; Jishun Li; Yanli Wei; Huan Liu; Yayu Wang; Shuheng Gan; Matthew D Denton Journal: Genome Biol Date: 2020-04-06 Impact factor: 13.583
Authors: Víctor M González-Mendoza; M E Sánchez-Sandoval; Lizbeth A Castro-Concha; S M Teresa Hernández-Sotomayor Journal: Plants (Basel) Date: 2021-05-04