| Literature DB >> 33494011 |
Ana Cláudia Camargo Miranda1, Ana Claudia Ranucci Durante2, Leonardo Lima Fuscaldi2, Angélica Bueno Barbezan3, Cilene Rebouças de Lima4, Efrain Perini3, Elaine Bortoleti de Araújo3.
Abstract
In the present work, the radioimmunoconjugates 111In-DTPA-trastuzumab and 177Lu-DOTA-trastuzumab were evaluated regarding the influence of the chelating agents on the physical-chemical parameters and human epidermal growth factor receptor 2 (HER2) tumor cell binding. Data showed that both chelating agents, at predetermined molar ratios (antibody:chelator - 1:10 and 1:20), did not influence the immunoconjugates integrity, the radiolabeling process and the radiolabeled antibodies stability. However, differences were observed in the lipophilic feature between DOTA and DTPA radioimmunoconjugates and in the specific binding to SK-BR-3 tumor cells (HER2 positive). Therefore, this study showed the importance of assessing the influence of chelating agents and their molar ratios in the development process of radioimmunoconjugates.Entities:
Keywords: (111)In-DTPA-trastuzumab; (177)Lu-DOTA-trastuzumab; Antibody:chelator molar ratio; Radioimmunoconjugate; Trastuzumab
Year: 2021 PMID： 33494011 DOI： 10.1016/j.bmc.2021.115996
Source DB: PubMed Journal: Bioorg Med Chem ISSN： 0968-0896 Impact factor: 3.641