MOTIVATION: A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. RESULTS: The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
MOTIVATION: A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. RESULTS: The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
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Authors: Felix Breden; Eline T Luning Prak; Bjoern Peters; Florian Rubelt; Chaim A Schramm; Christian E Busse; Jason A Vander Heiden; Scott Christley; Syed Ahmad Chan Bukhari; Adrian Thorogood; Frederick A Matsen Iv; Yariv Wine; Uri Laserson; David Klatzmann; Daniel C Douek; Marie-Paule Lefranc; Andrew M Collins; Tania Bubela; Steven H Kleinstein; Corey T Watson; Lindsay G Cowell; Jamie K Scott; Thomas B Kepler Journal: Front Immunol Date: 2017-11-01 Impact factor: 8.786