Literature DB >> 3349087

Enzymatic synthesis of p-nitrophenyl N,N',N'',N'',N''''-pentaacetyl-beta-chitopentaoside in water-methanol system; significance as a substrate for lysozyme assay.

T Usui1, Y Hayashi, F Nanjo, Y Ishido.   

Abstract

Transchitooligosylation from (GlcNAc)5 to the 4-position of PNP-GlcNAc was efficiently induced through lysozyme catalysis in an aqueous solution containing methanol with a high concentration. Use of the aqueous methanol system in this reaction not only guaranteed solubility of PNP-GlcNAc substrate, but also resulted in a remarkable increase in PNP-(GlcNAc)5 production. PNP-(GlcNAc)5 was substrate for lysozyme assay compared with PNP-(GlcNAc)4.

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Year:  1988        PMID： 3349087

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  6 in total

Review 1.  What's new in chitinase research?

Authors:  J Flach; P E Pilet; P Jollès
Journal:  Experientia       Date:  1992-08-15

2.  A novel transition-state analogue for lysozyme, 4-O-β-tri-N-acetylchitotriosyl moranoline, provided evidence supporting the covalent glycosyl-enzyme intermediate.

Authors:  Makoto Ogata; Naoyuki Umemoto; Takayuki Ohnuma; Tomoyuki Numata; Akari Suzuki; Taichi Usui; Tamo Fukamizo
Journal:  J Biol Chem       Date:  2013-01-09       Impact factor: 5.157

3.  Synthesis of Gal beta 1-3GlcNAc and Gal beta 1-3GlcNAc beta-SEt by an enzymatic method comprising the sequential use of beta-galactosidases from bovine testes and Escherichia coli.

Authors:  L Hedbys; E Johansson; K Mosbach; P O Larsson; A Gunnarsson; S Svensson; H Lönn
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

4.  Enzymic synthesis of the trisaccharide core region of the carbohydrate chain of N-glycoprotein.

Authors:  T Usui; M Suzuki; T Sato; H Kawagishi; K Adachi; H Sano
Journal:  Glycoconj J       Date:  1994-04       Impact factor: 2.916

5.  Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost.

Authors:  K Sakai; M Narihara; Y Kasama; M Wakayama; M Moriguchi
Journal:  Appl Environ Microbiol       Date:  1994-08       Impact factor: 4.792

6.  Enzymic synthesis of lacto-N-triose II and its positional analogues.

Authors:  Y Matahira; A Tashiro; T Sato; H Kawagishi; T Usui
Journal:  Glycoconj J       Date:  1995-10       Impact factor: 2.916
  6 in total

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