Literature DB >> 33490438

Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples.

Ajit Kumar1,2, Mirza Ghazanfar Ullah Ghazi1, Bhim Singh1, Syed Ainul Hussain3, Dinesh Bhatt2, Sandeep Kumar Gupta1.   

Abstract

We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301-1599 bp DNA fragments in various combinations. The method described may assist in the sequencing of either complete gene from moderate to good quality DNA or shorter fragment from degraded DNA.
© 2017 Wildlife Institute of India. Published by Informa UK Limited, trading as Taylor & Francis Group.

Entities:  

Keywords:  Mitochondrial cytochrome c oxidase I gene; PCR amplification; non-invasive and decomposed samples; ungulate species

Year:  2017        PMID： 33490438      PMCID： PMC7800399          DOI： 10.1080/23802359.2016.1247672

Source DB:  PubMed          Journal:  Mitochondrial DNA B Resour        ISSN： 2380-2359            Impact factor:   0.658


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