Literature DB >> 33486739

Eosinophil Shape Change and Secretion.

Sergei I Ochkur1, Elizabeth A Jacobsen1, Paige Lacy2.   

Abstract

The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor (PAF) and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an ELISA technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

Entities:  

Keywords:  Chemokine; Chemotaxis; Confocal microscopy; Degranulation; Eosinophil peroxidase; Eotaxin; Platelet-activating factor; Secretion

Year:  2021        PMID： 33486739     DOI： 10.1007/978-1-0716-1095-4_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

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Authors:  Kimberly D Dyer; Caroline M Percopo; Zhihui Xie; Zhao Yang; John Dongil Kim; Francis Davoine; Paige Lacy; Kirk M Druey; Redwan Moqbel; Helene F Rosenberg
Journal:  J Immunol       Date:  2010-04-26       Impact factor: 5.422

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Review 3.  The eosinophil.

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Journal:  Annu Rev Immunol       Date:  2006       Impact factor: 28.527

Review 4.  Eosinophils: biological properties and role in health and disease.

Authors:  Simon P Hogan; Helene F Rosenberg; Redwan Moqbel; Simon Phipps; Paul S Foster; Paige Lacy; A Barry Kay; Marc E Rothenberg
Journal:  Clin Exp Allergy       Date:  2008-04-01       Impact factor: 5.018

5.  Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs.

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Journal:  Proc Natl Acad Sci U S A       Date:  1998-12-22       Impact factor: 11.205

6.  Agonist activation of f-actin-mediated eosinophil shape change and mediator release is dependent on Rac2.

Authors:  Paige Lacy; Lian Willetts; John D Kim; Andrea N Lo; Bon Lam; Emily I Maclean; Redwan Moqbel; Marc E Rothenberg; Nives Zimmermann
Journal:  Int Arch Allergy Immunol       Date:  2011-05-16       Impact factor: 2.749

7.  Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia.

Authors:  E A Garcia-Zepeda; M E Rothenberg; R T Ownbey; J Celestin; P Leder; A D Luster
Journal:  Nat Med       Date:  1996-04       Impact factor: 53.440

8.  Mechanisms involved in eosinophil migration. Platelet-activating factor-induced chemotaxis and interleukin-5-induced chemokinesis are mediated by different signals.

Authors:  R C Schweizer; B A van Kessel-Welmers; R A Warringa; T Maikoe; J A Raaijmakers; J W Lammers; L Koenderman
Journal:  J Leukoc Biol       Date:  1996-03       Impact factor: 4.962

Review 9.  Mammalian Rho GTPases: new insights into their functions from in vivo studies.

Authors:  Sarah J Heasman; Anne J Ridley
Journal:  Nat Rev Mol Cell Biol       Date:  2008-09       Impact factor: 94.444

Review 10.  Eosinophils: changing perspectives in health and disease.

Authors:  Helene F Rosenberg; Kimberly D Dyer; Paul S Foster
Journal:  Nat Rev Immunol       Date:  2012-11-16       Impact factor: 53.106
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