| Literature DB >> 33483492 |
Yashavanth Shaan Lakshmanappa1, Sonny R Elizaldi1,2, Jamin W Roh1,2, Brian A Schmidt1, Timothy D Carroll3, Kourtney D Weaver4, Justin C Smith4, Anil Verma1, Jesse D Deere3, Joseph Dutra3, Mars Stone5,6, Sergej Franz6, Rebecca Lee Sammak3, Katherine J Olstad3, J Rachel Reader3,7, Zhong-Min Ma3, Nancy K Nguyen1, Jennifer Watanabe3, Jodie Usachenko3, Ramya Immareddy3, JoAnn L Yee3, Daniela Weiskopf8, Alessandro Sette8,9, Dennis Hartigan-O'Connor3,10, Stephen J McSorley1,11, John H Morrison3,12, Nam K Tran13, Graham Simmons5,6, Michael P Busch5,6, Pamela A Kozlowski4, Koen K A Van Rompay14,15, Christopher J Miller16,17,18, Smita S Iyer19,20,21.
Abstract
CD4 T follicular helper (Tfh) cells are important for the generation of durable and specific humoral protection against viral infections. The degree to which SARS-CoV-2 infection generates Tfh cells and stimulates the germinal center (GC) response is an important question as we investigate vaccine induced immunity against COVID-19. Here, we report that SARS-CoV-2 infection in rhesus macaques, either infused with convalescent plasma, normal plasma, or receiving no infusion, resulted in transient accumulation of pro-inflammatory monocytes and proliferating Tfh cells with a Th1 profile in peripheral blood. CD4 helper cell responses skewed predominantly toward a Th1 response in blood, lung, and lymph nodes. SARS-CoV-2 Infection induced GC Tfh cells specific for the SARS-CoV-2 spike and nucleocapsid proteins, and a corresponding early appearance of antiviral serum IgG antibodies. Collectively, the data show induction of GC responses in a rhesus model of mild COVID-19.Entities:
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Year: 2021 PMID： 33483492 PMCID： PMC7822826 DOI： 10.1038/s41467-020-20642-x
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919