Yi Gao1,2, Yanfeng Wang3, Xiaofei Wang4, Changan Zhao5, Fenghui Wang1,2, Juan Du1,2, Huahua Zhang1,2, Haiyan Shi1,2, Yun Feng1,2, Dan Li1,2, Jing Yan1,2, Yan Yao1,2, Weihong Hu1,2, Ruxin Ding1,2, Mengjie Zhang1,2, Lumin Wang6, Chen Huang7, Jing Zhang8,9. 1. Department of Cell Biology and Genetics, Medical College of Yan'an University, Yan'an, 716000, Shaanxi, China. 2. Yan'an Key Laboratory of Chronic Disease Prevention and Research, Yan'an, 716000, Shaanxi, China. 3. Department of Medical Genetic and Cell Biology, Ningxia Medical University, Yinchuan, 750004, China. 4. Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China. 5. Department of Pathology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, China. 6. Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China. wanglumin1@xjtu.edu.cn. 7. Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China. hchen@xjtu.edu.cn. 8. Department of Cell Biology and Genetics, Medical College of Yan'an University, Yan'an, 716000, Shaanxi, China. yadxzj@163.com. 9. Yan'an Key Laboratory of Chronic Disease Prevention and Research, Yan'an, 716000, Shaanxi, China. yadxzj@163.com.
Abstract
BACKGROUND: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. METHODS: Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. RESULTS: The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. CONCLUSIONS: These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.
BACKGROUND: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. METHODS: Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. RESULTS: The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. CONCLUSIONS: These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.
Authors: Manfred P Lutz; John R Zalcberg; Michel Ducreux; Antoine Adenis; William Allum; Daniela Aust; Fatima Carneiro; Heike I Grabsch; Pierre Laurent-Puig; Florian Lordick; Markus Möhler; Stefan Mönig; Radka Obermannova; Guillaume Piessen; Angela Riddell; Christoph Röcken; Franco Roviello; Paul Magnus Schneider; Stefan Seewald; Elizabeth Smyth; Eric van Cutsem; Marcel Verheij; Anna Dorothea Wagner; Florian Otto Journal: Eur J Cancer Date: 2019-03-15 Impact factor: 9.162
Authors: Alessio Biagioni; Anastasia Chillà; Mario Del Rosso; Gabriella Fibbi; Francesca Scavone; Elena Andreucci; Silvia Peppicelli; Francesca Bianchini; Lido Calorini; Anna Li Santi; Pia Ragno; Francesca Margheri; Anna Laurenzana Journal: Front Oncol Date: 2021-05-14 Impact factor: 6.244