Literature DB >> 33482637

A human brain test bed for research in large vessel occlusion stroke.

Daniel Gebrezgiabhier1,2, Yang Liu3,4, Adithya S Reddy1, Evan Davis3, Yihao Zheng3,5, Albert J Shih3, Aditya S Pandey1, Luis E Savastano1,6.   

Abstract

OBJECTIVE: Endovascular removal of emboli causing large vessel occlusion (LVO)-related stroke utilizing suction catheter and/or stent retriever technologies or thrombectomy is a new standard of care. Despite high recanalization rates, 40% of stroke patients still experience poor neurological outcomes as many cases cannot be fully reopened after the first attempt. The development of new endovascular technologies and techniques for mechanical thrombectomy requires more sophisticated testing platforms that overcome the limitations of phantom-based simulators. The authors investigated the use of a hybrid platform for LVO stroke constructed with cadaveric human brains.
METHODS: A test bed for embolic occlusion of cerebrovascular arteries and mechanical thrombectomy was developed with cadaveric human brains, a customized hydraulic system to generate physiological flow rate and pressure, and three types of embolus analogs (elastic, stiff, and fragment-prone) engineered to match mechanically and phenotypically the emboli causing LVO strokes. LVO cases were replicated in the anterior and posterior circulation, and thrombectomy was attempted using suction catheters and/or stent retrievers.
RESULTS: The test bed allowed radiation-free visualization of thrombectomy for LVO stroke in real cerebrovascular anatomy and flow conditions by transmural visualization of the intraluminal elements and procedures. The authors were able to successfully replicate 105 LVO cases with 184 passes in 12 brains (51 LVO cases and 82 passes in the anterior circulation, and 54 LVO cases and 102 passes in the posterior circulation). Observed recanalization rates in this model were graded using a Recanalization in LVO (RELVO) scale analogous to other measures of recanalization outcomes in clinical use.
CONCLUSIONS: The human brain platform introduced and validated here enables the analysis of artery-embolus-device interaction under physiological hemodynamic conditions within the unmodified complexity of the cerebral vasculature inside the human brain.

Entities:  

Keywords:  cadaveric model; embolus; large vessel occlusion; stroke; thrombectomy; vascular disorders

Year:  2021        PMID: 33482637     DOI: 10.3171/2020.7.JNS202278

Source DB:  PubMed          Journal:  J Neurosurg        ISSN: 0022-3085            Impact factor:   5.115


  4 in total

1.  Arterial Collapse during Thrombectomy for Stroke: Clinical Evidence and Experimental Findings in Human Brains and In Vivo Models.

Authors:  Y Liu; D Gebrezgiabhier; Y Zheng; A J Shih; N Chaudhary; A S Pandey; J L A Larco; S I Madhani; M Abbasi; A H Shahid; R A Quinton; R Kadirvel; W Brinjikji; D F Kallmes; L E Savastano
Journal:  AJNR Am J Neuroradiol       Date:  2022-01-13       Impact factor: 3.825

2.  Novel Human Acute Ischemic Stroke Blood Clot Analogs for In Vitro Thrombectomy Testing.

Authors:  S T Fitzgerald; Y Liu; D Dai; O M Mereuta; M Abbasi; J L A Larco; A S Douglas; D F Kallmes; L Savastano; K M Doyle; W Brinjikji
Journal:  AJNR Am J Neuroradiol       Date:  2021-04-08       Impact factor: 4.966

Review 3.  Preclinical testing platforms for mechanical thrombectomy in stroke: a review on phantoms, in-vivo animal, and cadaveric models.

Authors:  Yang Liu; Mehdi Abbasi; Jorge L Arturo Larco; Ramanathan Kadirvel; David F Kallmes; Waleed Brinjikji; Luis Savastano
Journal:  J Neurointerv Surg       Date:  2021-03-15       Impact factor: 8.572

4.  Novel synthetic clot analogs for in-vitro stroke modelling.

Authors:  Helena Guerreiro; Nadine Wortmann; Thomas Andersek; Tuan N Ngo; Andreas M Frölich; Dieter Krause; Jens Fiehler; Anna A Kyselyova; Fabian Flottmann
Journal:  PLoS One       Date:  2022-09-09       Impact factor: 3.752

  4 in total

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