| Literature DB >> 33481228 |
Noah Steinfeld1,2, Sai Srinivas Panapakkam Giridharan1, Emily J Kauffman1, Lois S Weisman3,4.
Abstract
Phosphoinositide (PPI) lipids are a crucial class of low-abundance signaling molecules that regulate many processes within cells. Methods that enable simultaneous detection of all PPI lipid species provide a wholistic snapshot of the PPI profile of cells, which is critical for probing PPI biology. Here we describe a method for the simultaneous measurement of cellular PPI levels by metabolically labeling yeast or mammalian cells with myo-3H-inositol, extracting radiolabeled glycerophosphoinositides, and separating lipid species on an anion exchange column via HPLC.Entities:
Keywords: PI3,4,5P3; PI3,4P2; PI3,5P2; PI3P; PI4,5P2; PI4P; PI5P; Phosphatidylinositol; Phosphoinositide lipids; PtdIns; Radioactive metabolic labeling
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Year: 2021 PMID： 33481228 PMCID： PMC8059495 DOI： 10.1007/978-1-0716-1142-5_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745