Literature DB >> 33480256

Genome Mining Reveals a Multiproduct Sesterterpenoid Biosynthetic Gene Cluster in Aspergillus ustus.

Jingjing Guo1, You-Sheng Cai2, Fangcai Cheng1, Chenjie Yang1, Wenqi Zhang1, Wulin Yu1, Jingjing Yan1, Zixin Deng1, Kui Hong1.   

Abstract

Genome mining of Aspergillus ustus 094102 enabled the discovery of a multiproduct bifunctional terpene synthase (BTS), AuAS. Heterologous expression of AuAS led to the discovery of five new sesterterpenes, and coexpression of the upstream CYP450 monooxygenase (AuAP450) generated four new sesterterpene alcohols. Additionally, aspergilol A showed cytotoxic activities against MCF-7, MDA-MB231, and HepG2 cancer cells (IC50 21.20-48.76 μM), and aspergilol B exhibited a cytotoxic effect on MCF-7 cells (IC50 27.41 μM).

Entities:  

Year:  2021        PMID： 33480256     DOI： 10.1021/acs.orglett.0c03996

Source DB:  PubMed          Journal:  Org Lett        ISSN： 1523-7052            Impact factor:   6.005


  9 in total

1.  Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus Amphichordafelina SYSU-MS7908.

Authors:  Siwen Yuan; Litong Chen; Qilin Wu; Minghua Jiang; Heng Guo; Zhibo Hu; Senhua Chen; Lan Liu; Zhizeng Gao
Journal:  Mar Drugs       Date:  2022-04-27       Impact factor: 6.085

2.  Schultriene and nigtetraene: two sesterterpenes characterized from pathogenetic fungi via genome mining approach.

Authors:  Lan Jiang; Huanting Yang; Xue Zhang; Xiaoying Li; Kangjie Lv; Weiyan Zhang; Guoliang Zhu; Chengwei Liu; Yongheng Wang; Tom Hsiang; Lixin Zhang; Xueting Liu
Journal:  Appl Microbiol Biotechnol       Date:  2022-08-30       Impact factor: 5.560

3.  The Biosynthesis and Transport of Ophiobolins in Aspergillus ustus 094102.

Authors:  Jingjing Yan; Jiamin Pang; Jianjia Liang; Wulin Yu; Xuequn Liao; Ayikaimaier Aobulikasimu; Xinrui Yi; Yapeng Yin; Zixin Deng; Kui Hong
Journal:  Int J Mol Sci       Date:  2022-02-08       Impact factor: 5.923

4.  Optimization of Protoplast Preparation and Establishment of Genetic Transformation System of an Arctic-Derived Fungus Eutypella sp.

Authors:  Yaodong Ning; Bo Hu; Haobing Yu; Xiaoyu Liu; Binghua Jiao; Xiaoling Lu
Journal:  Front Microbiol       Date:  2022-04-06       Impact factor: 6.064

5.  Enzymatic Synthesis of Variediene Analogs.

Authors:  Lin-Fu Liang; Jeroen S Dickschat
Journal:  Chemistry       Date:  2022-02-09       Impact factor: 5.020

6.  Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae.

Authors:  Lan Jiang; Kangjie Lv; Guoliang Zhu; Zhi Lin; Xue Zhang; Cuiping Xing; Huanting Yang; Weiyan Zhang; Zhixin Wang; Chengwei Liu; Xudong Qu; Tom Hsiang; Lixin Zhang; Xueting Liu
Journal:  Synth Syst Biotechnol       Date:  2022-08-23

7.  Mechanism of the Bifunctional Multiple Product Sesterterpene Synthase AcAS from Aspergillus calidoustus.

Authors:  Zhiyang Quan; Anwei Hou; Bernd Goldfuss; Jeroen S Dickschat
Journal:  Angew Chem Int Ed Engl       Date:  2022-02-04       Impact factor: 16.823

8.  Identification and Characterization of a Cryptic Bifunctional Type I Diterpene Synthase Involved in Talaronoid Biosynthesis from a Marine-Derived Fungus.

Authors:  Peng Zhang; Guangwei Wu; Stephanie C Heard; Changshan Niu; Stephen A Bell; Fengli Li; Ying Ye; Yonghui Zhang; Jaclyn M Winter
Journal:  Org Lett       Date:  2022-09-20       Impact factor: 6.072

9.  Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.

Authors:  Rong Chen; Qidong Jia; Xin Mu; Ben Hu; Xiang Sun; Zixin Deng; Feng Chen; Guangkai Bian; Tiangang Liu
Journal:  Proc Natl Acad Sci U S A       Date:  2021-07-20       Impact factor: 11.205
  9 in total

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