Literature DB >> 334785

A non-enzymic procdure for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)ethylene glycol(MHPG sulphate) in human urine using stable isotope dilution and gas chromatography-mass spectrometry.

S Murray, T A Baillie, D S Davies.   

Abstract

A method is described for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)-ethylene glycol (MHPG sulphate) in human urine, based on selected ion monitoring gas chromatography--mass spectrometry and using a specifically deuterium-labelled analogue of MHPG sulphate as internal standard. The procedure involves extraction of the urine sample on Amberlite XAD-2, followed by isolation of MHPG sulphate by column chromatography on Sephadex LH-20. Cleavage of the sulphate conjugate and formation of the MHPG tris(trifluoroacetate) derivative are carried out in a one-step reaction, without recourse to enzymic hydrolysis.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 334785     DOI： 10.1016/s0378-4347(00)81310-9

Source DB:  PubMed          Journal:  J Chromatogr


  4 in total

Review 1.  Urinary MHPG sulfate as a marker of central norepinephrine metabolism: a commentary.

Authors:  L Peyrin
Journal:  J Neural Transm Gen Sect       Date:  1990

2.  Proceedings of the British Pharmacological Society. 17th-19th December, 1979: University of London (Institute of Education).

Authors: 
Journal:  Br J Pharmacol       Date:  1980-06       Impact factor: 8.739

3.  Stereoselective excretion of (3-methoxy-4-sulphooxyphenyl)ethylene glycol (MHPG sulphate) in the dog.

Authors:  S Murray; A R Boobis; H Frank; T A Baillie; D S Davies
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1980-10       Impact factor: 3.000

4.  Free and conjugated 3-methoxy-4-hydroxyphenylglycol in human urine: peripheral origin of glucuronide.

Authors:  L Peyrin; J M Pequignot
Journal:  Psychopharmacology (Berl)       Date:  1983       Impact factor: 4.530
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.