Sharafaldin Al-Musawi1, Sumayah Ibraheem2, Salih Abdul Mahdi1, Salim Albukhaty3, Adawiya J Haider4, Afraa Ali Kadhim5, Kadhim Ali Kadhim6, Haitham Ali Kadhim7, Hassan Al-Karagoly8. 1. Faculty of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq. 2. Al_kindy College of Medicine, University of Baghdad, Baghdad 10071, Iraq. 3. Department of Chemistry, College of Science, University of Misan, Maysan 62001, Iraq. 4. Applied Science Department/Laser Science and Technology Branch, University of Technology, Baghdad 10066, Iraq. 5. Department of Biology, College of Science, Mustansiriyah University, Baghdad 14022, Iraq. 6. Department of Pharmacy, Al-Yarmouk University College, Baghdad 56001, Iraq. 7. Iraq Ministry of Health, Medico Legal Directorate, Baghdad 10011, Iraq. 8. Department of Internal and Preventive Medicine, Veterinary Medicine College, University of Al-Qadisiyah, Diwaniyah 58002, Iraq.
Abstract
BACKGROUND: Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently. METHODS: A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1 testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs). RESULTS: This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.
BACKGROUND: Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently. METHODS: A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs). RESULTS: This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.
Authors: Udai Banerji; Emma J Dean; J Alejandro Pérez-Fidalgo; Gerald Batist; Philippe L Bedard; Benoit You; Shannon N Westin; Peter Kabos; Michelle D Garrett; Mathew Tall; Helen Ambrose; J Carl Barrett; T Hedley Carr; S Y Amy Cheung; Claire Corcoran; Marie Cullberg; Barry R Davies; Elza C de Bruin; Paul Elvin; Andrew Foxley; Peter Lawrence; Justin P O Lindemann; Rhiannon Maudsley; Martin Pass; Vicky Rowlands; Paul Rugman; Gaia Schiavon; James Yates; Jan H M Schellens Journal: Clin Cancer Res Date: 2017-10-24 Impact factor: 12.531