Yiyun Cai1,2,3, Runkai Wang1,2, Pinhua Rao1, Baichun Wu3, Lili Yan1, Lijiang Hu1,4, Sangsook Park5, Moonhee Ryu6, Xiaoya Zhou1. 1. School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. 2. College of Civil Engineering, Kashgar University, Kashgar 844006, China. 3. State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China. 4. Anji Guoqian Environmental Technology Co., Ltd., Huzhou 313000, China. 5. Department of Environmental Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea. 6. Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.
Abstract
Biodegradation has been considered as an ideal technique for total petroleum hydrocarbon (TPH) contamination, but its efficiency is limited by its application in the field. Herein, an original TPH-degrading strain, SCYY-5, was isolated from contaminated oil sludge and identified as Acinetobacter sp. by 16S rDNA sequence analysis. The biological function of the isolate was investigated by heavy metal tolerance, carbon, and nitrogen source and degradation tests. To enhance its biodegradation efficiency, the response surface methodology (RSM) based on a function model was adopted to investigate and optimize the strategy of microbial and environmental variables for TPH removal. Furthermore, the performance of the system increased to 79.94% with the further addition of extra nutrients, suggesting that the RSM and added nutrients increased the activity of bacteria to meet the needs of the co-metabolism matrix during growth or degradation. These results verified that it is feasible to adopt the optimal strategy of combining bioremediation with RSM to improve the biodegradation efficiency, for contaminated oil sludge.
Biodegradation has been considered as an ideal technique for total class="Chemical">petroleum hydrocarbon (n class="Chemical">TPH) contamination, but its efficiency is limited by its application in the field. Herein, an original TPH-degrading strain, SCYY-5, was isolated from contaminated oil sludge and identified as Acinetobacter sp. by 16S rDNA sequence analysis. The biological function of the isolate was investigated by heavy metal tolerance, carbon, and nitrogen source and degradation tests. To enhance its biodegradation efficiency, the response surface methodology (RSM) based on a function model was adopted to investigate and optimize the strategy of microbial and environmental variables for TPH removal. Furthermore, the performance of the system increased to 79.94% with the further addition of extra nutrients, suggesting that the RSM and added nutrients increased the activity of bacteria to meet the needs of the co-metabolism matrix during growth or degradation. These results verified that it is feasible to adopt the optimal strategy of combining bioremediation with RSM to improve the biodegradation efficiency, for contaminated oil sludge.
Authors: Mengjie Hu; Feifan Zhang; Gaoyuan Li; Haihua Ruan; Xinhao Li; Lei Zhong; Guanyi Chen; Yichao Rui Journal: Int J Environ Res Public Health Date: 2022-09-19 Impact factor: 4.614