| Literature DB >> 33476009 |
Kathleen Cantow1, Mechthild Ladwig-Wiegard2, Bert Flemming1, Andreas Pohlmann3,4, Thoralf Niendorf3, Erdmann Seeliger5.
Abstract
Renal tissue hypoperfusion and hypoxia are early key elements in the pathophysiology of acute kidney injury of various origins, and may also promote progression from acute injury to chronic kidney disease. Here we describe methods to study control of renal hemodynamics and tissue oxygenation by means of invasive probes in anesthetized rats. Step-by-step protocols are provided for two setups, one for experiments in laboratories for integrative physiology and the other for experiments within small-animal magnetic resonance scanners.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by a separate chapter describing the basic concepts of quantitatively assessing renal perfusion and oxygenation with invasive probes.Entities:
Keywords: In vivo methods; Magnetic resonance imaging (MRI); Rats; Renal hemodynamics and oxygenation
Year: 2021 PMID： 33476009 DOI： 10.1007/978-1-0716-0978-1_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745