Literature DB >> 33475807

[Mycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR].

Torsten Goldmann1,2, Doris Hillemann3, Florian Maurer3,4, Barbara Kalsdorf5, Rosemarie Krupar1,6, Florian Stellmacher7, Sven Perner1,2,6.   

Abstract

The detection of Mycobacterium tuberculosis complex DNA by PCR using formalin-fixed paraffin-embedded material has become an integral part of molecular-pathological diagnostics. We describe an approach that enables the detection of contamination by using Mycobacterium szulgai as a positive control, contributing to the reduction of false-positive results.

Entities:  

Keywords:  DNA sequence analysis; Molecular pathology; Mycobacteria; Polymerase chain reaction; Quality control

Year:  2021        PMID: 33475807     DOI: 10.1007/s00292-021-00912-1

Source DB:  PubMed          Journal:  Pathologe        ISSN: 0172-8113            Impact factor:   1.011


  1 in total

1.  Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues.

Authors:  Christiane Schewe; Torsten Goldmann; Marianne Grosser; Albert Zink; Karsten Schlüns; Stefan Pahl; Timo Ulrichs; Stefan H E Kaufmann; Andreas Nerlich; Gustavo B Baretton; Manfred Dietel; Ekkehard Vollmer; Iver Petersen
Journal:  Virchows Arch       Date:  2005-06-21       Impact factor: 4.064

  1 in total

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