Simone La Padula1,2,3,4, Barbara Hersant5, Chiara Pizza5, Christophe Chesné6, Agnes Jamin6, Ismail Ben Mosbah6,7, Concetta Errico5, Francesco D'Andrea8, Umberto Rega8, Paolo Persichetti9, Jean Paul Meningaud5. 1. Department of Plastic, Reconstructive and Maxillo facial Surgery, Henri Mondor Hospital, University Paris XII, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France. drsimonelapadula@gmail.com. 2. Department of Plastic and Reconstructive Surgery, Università Campus Biomedico di Roma, Via Alvaro del Portillo, 21, 00128, Roma, Italy. drsimonelapadula@gmail.com. 3. Department of Plastic and Reconstructive Surgery, Università degli studi di Napoli Federico II, Via Pansini 5, 80131, Napoli, Italy. drsimonelapadula@gmail.com. 4. , 50 rue Saint Sébastien, Paris, 75011, France. drsimonelapadula@gmail.com. 5. Department of Plastic, Reconstructive and Maxillo facial Surgery, Henri Mondor Hospital, University Paris XII, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France. 6. Biopredic International, Parc d'activité de la Bretèche Bâtiment A4, 35760, Saint Grégoire, France. 7. IMRB - Institut Mondor de Recherche Biomédicale, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France. 8. Department of Plastic and Reconstructive Surgery, Università degli studi di Napoli Federico II, Via Pansini 5, 80131, Napoli, Italy. 9. Department of Plastic and Reconstructive Surgery, Università Campus Biomedico di Roma, Via Alvaro del Portillo, 21, 00128, Roma, Italy.
Abstract
INTRODUCTION: Striae distensae (SD) appear clinically as parallel striae, lying perpendicular to the tension lines of the skin. SD evolve into two clinical phases, an initial inflammatory phase in which they are called "striae rubrae" (SR) and a chronic phase in which they are called striae albae (SA). Fibroblasts seem to play a key role in the pathogenesis of stretch marks. This study was aimed at describing and analyzing stretch marks-derived fibroblasts (SMF), the differences between SR- and SA-derived fibroblasts (SRF, SAF), testing two treatments in vitro (sodium ascorbate and PrP) on SAF. MATERIAL AND METHODS: To characterize the SMF, the expression of alpha smooth muscle actin (alpha SMA) was investigated. Type I collagen expression was measured in SAF, before and after adding different PrP concentrations and sodium ascorbate in the culture medium. Results were processed through statistical analysis models using the Student's t-test. RESULTS: A significant increase in alpha SMA (P <0.001) was observed in SRF. SAF treated with PrP and sodium ascorbate showed a resumption of their metabolic activity by an increase in collagen type I production and cell proliferation. After 24 h of incubation with PrP 1% and PrP 5% + sodium ascorbate, cell viability was increased by 140% and 151% and by 156 and 178% after 48 h, respectively, compared to the control. CONCLUSION: Our study shows that a biologically mediated improvement in SMF metabolic activity is possible. Our promising results require further trials to be able to confirm the reproducibility of this combined treatment, particularly in vivo. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable.
INTRODUCTION: Striae distensae (SD) appear clinically as parallel striae, lying perpendicular to the tension lines of the skin. SD evolve into two clinical phases, an initial inflammatory phase in which they are called "striae rubrae" (SR) and a chronic phase in which they are called striae albae (SA). Fibroblasts seem to play a key role in the pathogenesis of stretch marks. This study was aimed at describing and analyzing stretch marks-derived fibroblasts (SMF), the differences between SR- and SA-derived fibroblasts (SRF, SAF), testing two treatments in vitro (sodium ascorbate and PrP) on SAF. MATERIAL AND METHODS: To characterize the SMF, the expression of alpha smooth muscle actin (alpha SMA) was investigated. Type I collagen expression was measured in SAF, before and after adding different PrP concentrations and sodium ascorbate in the culture medium. Results were processed through statistical analysis models using the Student's t-test. RESULTS: A significant increase in alpha SMA (P <0.001) was observed in SRF. SAF treated with PrP and sodium ascorbate showed a resumption of their metabolic activity by an increase in collagen type I production and cell proliferation. After 24 h of incubation with PrP 1% and PrP 5% + sodium ascorbate, cell viability was increased by 140% and 151% and by 156 and 178% after 48 h, respectively, compared to the control. CONCLUSION: Our study shows that a biologically mediated improvement in SMF metabolic activity is possible. Our promising results require further trials to be able to confirm the reproducibility of this combined treatment, particularly in vivo. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable.
Authors: Simone La Padula; Rosita Pensato; Francesco D'Andrea; Ludovica de Gregorio; Concetta Errico; Umberto Rega; Luigi Canta; Chiara Pizza; Giovanni Roccaro; Raphaelle Billon; Endri Dibra; Jean Paul Meningaud; Barbara Hersant Journal: J Clin Med Date: 2022-06-16 Impact factor: 4.964
Authors: Simone La Padula; Rosita Pensato; Antonio Zaffiro; Oana Hermeziu; Francesco D'Andrea; Chiara Pizza; Jean Paul Meningaud; Barbara Hersant Journal: J Clin Med Date: 2022-04-13 Impact factor: 4.964
Authors: Zhouxiao Li; Thilo Ludwig Schenck; Riccardo Enzo Giunta; Lucas Etzel; Konstantin Christoph Koban Journal: J Clin Med Date: 2022-07-11 Impact factor: 4.964
Authors: Simone La Padula; Rosita Pensato; Chiara Pizza; Edoardo Coiante; Giovanni Roccaro; Benedetto Longo; Francesco D'Andrea; Francesco Saverio Wirz; Barbara Hersant; Jean Paul Meningaud Journal: J Clin Med Date: 2022-09-28 Impact factor: 4.964