Complete mitochondrial genome sequence of Zhuxiang chicken (Gallus gallus. domesticus) and its phylogenetic analysis from D-loop region.

Zhuxiang chicken is a valuable chicken breed in the southwest of China. In this study, we firstly obtained the complete mitochondrial genome sequence of Zhuxiang chicken using PCR amplification, sequencing and assembling. The total length of the mitochondrial genome was 16,789 bp, based on composition of 30.24% for A, 23.71% for T, 32.54% for C, 13.51% for G, contained a D-loop region (non-coding control region), 2 ribosomal RNA genes, 13 protein-coding genes and 22 transfer RNA. This research was significant for mtDNA structure and provided phylogenetic analysis of D-loop sequence for carrying out Zhuxiang chicken germplasm resources protection, rational breeding, origin and evolution studies.