Mohammad S Al-Hwaiti1, Eid M Alsbou2, Ghassan Abu Sheikha3, Boulanouar Bakchiche4, Thu Huong Pham5, Raymond H Thomas5, Sanaa K Bardaweel6. 1. Department of Environmental Engineering Faculty of Engineering Al-Hussein Bin Talal University Ma'an Jordan. 2. Chemistry Department Faculty of Science Al-Hussein Bin Talal University Ma'an Jordan. 3. Department of Pharmacy Al-Zaytoonah University of Jordan Ma'an Jordan. 4. Laboratory of Process Engineering Faculty of Technology Laghouat University Laghouat Algeria. 5. School of Science and the Environment/Boreal Ecosystem Research Facility Grenfell Campus Memorial University of Newfoundland Corner Brook NL Canada. 6. Department of Pharmaceutical Sciences School of Pharmacy University of Jordan Amman Jordan.
Abstract
BACKGROUND: The chemical composition of Handal (Citrullus colocynthis L.) seed oil cultivated in Jordan deserts was characterized, and its bioactivity was evaluated. METHODS: The oil was extracted from the grinded seeds in 500 ml Soxhlet extractor for 24 hr using n-hexane, and the recovered fatty acids were methylated with methanolic-HCL. The fatty acid methyl esters (FAMEs) composition was analyzed using GC-MS and GC-FID. The anticancer activity associated with the oil was assessed against colon cancer cell lines (Caco-2 and HCT-116) and compared to its cytotoxicity on the human skin fibroblast. Multivariate analysis was used to determine relationship of the fatty acid composition with that of the anticancer activity. RESULTS: The results demonstrated that fatty acid composition of Citrullus colocynthis seed oil chiefly contains Linoleic acid, denoted as C18:2n6 (75%), followed by Palmitic acid C16:0 (8%), Stearic acid C18:0 (5%), and Oleic acid C18:1n9 (9%). It is demonstrated as an excellent source of essential fatty acids omega-6 (e.g., Linoleic acid), whereas omega-3 (e.g., α-Linolenic acid) and hydroxy polyunsaturated fatty acids are found at small level. Interestingly, the oil exhibited reasonable anticancer effects against colorectal cancer cell lines with IC50 values varying between 4 and 7 mg/ml. The correlation test revealed a relationship between the fatty acid composition and the effectiveness on treatments. CONCLUSIONS: Handal plant from Jordan appears to have very high level of Linoleic acid compared to other oils measured in different geographic locations and that there appears to be some anticancer activities associated with the fatty acid content of Handal seed oil.
BACKGROUND: The chemical composition of Handal (Citrullus colocynthis L.) seed oil cultivated in Jordan deserts was characterized, and its bioactivity was evaluated. METHODS: The oil was extracted from the grinded seeds in 500 ml Soxhlet extractor for 24 hr using n-hexane, and the recovered fatty acids were methylated with methanolic-HCL. The fatty acid methyl esters (FAMEs) composition was analyzed using GC-MS and GC-FID. The anticancer activity associated with the oil was assessed against colon cancer cell lines (Caco-2 and HCT-116) and compared to its cytotoxicity on the human skin fibroblast. Multivariate analysis was used to determine relationship of the fatty acid composition with that of the anticancer activity. RESULTS: The results demonstrated that fatty acid composition of Citrullus colocynthis seed oil chiefly contains Linoleic acid, denoted as C18:2n6 (75%), followed by Palmitic acid C16:0 (8%), Stearic acid C18:0 (5%), and Oleic acid C18:1n9 (9%). It is demonstrated as an excellent source of essential fatty acids omega-6 (e.g., Linoleic acid), whereas omega-3 (e.g., α-Linolenic acid) and hydroxy polyunsaturated fatty acids are found at small level. Interestingly, the oil exhibited reasonable anticancer effects against colorectal cancer cell lines with IC50 values varying between 4 and 7 mg/ml. The correlation test revealed a relationship between the fatty acid composition and the effectiveness on treatments. CONCLUSIONS: Handal plant from Jordan appears to have very high level of Linoleic acid compared to other oils measured in different geographic locations and that there appears to be some anticancer activities associated with the fatty acid content of Handal seed oil.
Authors: R Nmila; R Gross; H Rchid; M Roye; M Manteghetti; P Petit; M Tijane; G Ribes; Y Sauvaire Journal: Planta Med Date: 2000-06 Impact factor: 3.352
Authors: Abdullah I Hussain; Hassaan A Rathore; Munavvar Z A Sattar; Shahzad A S Chatha; Satyajit D Sarker; Anwar H Gilani Journal: J Ethnopharmacol Date: 2014-06-14 Impact factor: 4.360
Authors: Sanaa K Bardaweel; Mohammad M Hudaib; Khaled A Tawaha; Rasha M Bashatwah Journal: Evid Based Complement Alternat Med Date: 2015-07-28 Impact factor: 2.629
Authors: Mohamed I Alzarah; Fayez Althobiati; Ahmed O Abbas; Gamal M K Mehaisen; Nancy N Kamel Journal: Animals (Basel) Date: 2021-06-30 Impact factor: 2.752