Literature DB >> 33472065

Heat activates the AAA+ HslUV protease by melting an axial autoinhibitory plug.

Vladimir Baytshtok1, Xue Fei1, Tsai-Ting Shih1, Robert A Grant1, Justin C Santos1, Tania A Baker1, Robert T Sauer2.   

Abstract

At low temperatures, protein degradation by the AAA+ HslUV protease is very slow. New crystal structures reveal that residues in the intermediate domain of the HslU6 unfoldase can plug its axial channel, blocking productive substrate binding and subsequent unfolding, translocation, and degradation by the HslV12 peptidase. Biochemical experiments with wild-type and mutant enzymes support a model in which heat-induced melting of this autoinhibitory plug activates HslUV proteolysis.
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Entities:  

Keywords:  ATP-dependent degradation; heat shock locus; proteolytic regulation; temperature control

Mesh：

Substances：

Year:  2021        PMID： 33472065      PMCID： PMC7849044          DOI： 10.1016/j.celrep.2020.108639

Source DB:  PubMed          Journal:  Cell Rep            Impact factor:   9.423


  22 in total

1.  Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU.

Authors:  J Wang; J J Song; I S Seong; M C Franklin; S Kamtekar; S H Eom; C H Chung
Journal:  Structure       Date:  2001-11       Impact factor: 5.006

2.  EMAN2: an extensible image processing suite for electron microscopy.

Authors:  Guang Tang; Liwei Peng; Philip R Baldwin; Deepinder S Mann; Wen Jiang; Ian Rees; Steven J Ludtke
Journal:  J Struct Biol       Date:  2006-06-08       Impact factor: 2.867

Review 3.  AAA+ proteases: ATP-fueled machines of protein destruction.

Authors:  Robert T Sauer; Tania A Baker
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

4.  The ATP-dependent HslVU protease from Escherichia coli is a four-ring structure resembling the proteasome.

Authors:  M Rohrwild; G Pfeifer; U Santarius; S A Müller; H C Huang; A Engel; W Baumeister; A L Goldberg
Journal:  Nat Struct Biol       Date:  1997-02

5.  XDS.

Authors:  Wolfgang Kabsch
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22

6.  UCSF ChimeraX: Meeting modern challenges in visualization and analysis.

Authors:  Thomas D Goddard; Conrad C Huang; Elaine C Meng; Eric F Pettersen; Gregory S Couch; John H Morris; Thomas E Ferrin
Journal:  Protein Sci       Date:  2017-09-06       Impact factor: 6.725

7.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

8.  P22 Arc repressor: enhanced expression of unstable mutants by addition of polar C-terminal sequences.

Authors:  M E Milla; B M Brown; R T Sauer
Journal:  Protein Sci       Date:  1993-12       Impact factor: 6.725

9.  Features and development of Coot.

Authors:  P Emsley; B Lohkamp; W G Scott; K Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

10.  PHENIX: a comprehensive Python-based system for macromolecular structure solution.

Authors:  Paul D Adams; Pavel V Afonine; Gábor Bunkóczi; Vincent B Chen; Ian W Davis; Nathaniel Echols; Jeffrey J Headd; Li-Wei Hung; Gary J Kapral; Ralf W Grosse-Kunstleve; Airlie J McCoy; Nigel W Moriarty; Robert Oeffner; Randy J Read; David C Richardson; Jane S Richardson; Thomas C Terwilliger; Peter H Zwart
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22
View more
  1 in total

Review 1.  Checkpoints That Regulate Balanced Biosynthesis of Lipopolysaccharide and Its Essentiality in Escherichia coli.

Authors:  Gracjana Klein; Alicja Wieczorek; Martyna Szuster; Satish Raina
Journal:  Int J Mol Sci       Date:  2021-12-24       Impact factor: 5.923
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.