Literature DB >> 33471332

Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice.

Xu Tang1, Yiping Qi2,3, Yong Zhang4.   

Abstract

CRISPR-Cas9 and Cas12a (formerly Cpf1), RNA-guided DNA endonucleases found from adaptive immune system in prokaryotes, have been engineered and widely adopted as two of the most powerful genome editing systems in plants. Recently, we developed a single transcript unit (STU) CRISPR 2.0 toolbox for applications in plants, which contains two STU-Cas9 systems and one STU-Cas12a system. Here, we describe a detailed protocol about using the STU CRISPR 2.0 systems to achieve single and multiplex genome editing in rice.

Entities:  

Keywords:  CRISPR-Cas; Cas12a; Cas9; Csy4; Genome editing; Golden Gate; STU CRISPR 2.0; tRNA

Year:  2021        PMID： 33471332     DOI： 10.1007/978-1-0716-1068-8_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

Authors:  Bernd Zetsche; Jonathan S Gootenberg; Omar O Abudayyeh; Ian M Slaymaker; Kira S Makarova; Patrick Essletzbichler; Sara E Volz; Julia Joung; John van der Oost; Aviv Regev; Eugene V Koonin; Feng Zhang
Journal:  Cell       Date:  2015-09-25       Impact factor: 41.582

2.  Targeted genome modification of crop plants using a CRISPR-Cas system.

Authors:  Qiwei Shan; Yanpeng Wang; Jun Li; Yi Zhang; Kunling Chen; Zhen Liang; Kang Zhang; Jinxing Liu; Jianzhong Jeff Xi; Jin-Long Qiu; Caixia Gao
Journal:  Nat Biotechnol       Date:  2013-08       Impact factor: 54.908

3.  Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.

Authors:  Vladimir Nekrasov; Brian Staskawicz; Detlef Weigel; Jonathan D G Jones; Sophien Kamoun
Journal:  Nat Biotechnol       Date:  2013-08       Impact factor: 54.908

4.  The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA.

Authors:  Ines Fonfara; Hagen Richter; Majda Bratovič; Anaïs Le Rhun; Emmanuelle Charpentier
Journal:  Nature       Date:  2016-04-20       Impact factor: 49.962

5.  Cardiologists send up a trial balloon in new efforts to relieve heart failure.

Authors:  M F Goldsmith
Journal:  JAMA       Date:  1987-01-16       Impact factor: 56.272

6.  Segmental air plethysmography in the diagnosis of deep vein thrombosis.

Authors:  A B van Rijn; I Heller; J Van Zijl
Journal:  Surg Gynecol Obstet       Date:  1987-12

7.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

8.  Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.

Authors:  Jian-Feng Li; Julie E Norville; John Aach; Matthew McCormack; Dandan Zhang; Jenifer Bush; George M Church; Jen Sheen
Journal:  Nat Biotechnol       Date:  2013-08       Impact factor: 54.908

9.  Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.

Authors:  Bernd Zetsche; Matthias Heidenreich; Prarthana Mohanraju; Iana Fedorova; Jeroen Kneppers; Ellen M DeGennaro; Nerges Winblad; Sourav R Choudhury; Omar O Abudayyeh; Jonathan S Gootenberg; Wen Y Wu; David A Scott; Konstantin Severinov; John van der Oost; Feng Zhang
Journal:  Nat Biotechnol       Date:  2016-12-05       Impact factor: 54.908

10.  Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

Authors:  Baohui Chen; Luke A Gilbert; Beth A Cimini; Joerg Schnitzbauer; Wei Zhang; Gene-Wei Li; Jason Park; Elizabeth H Blackburn; Jonathan S Weissman; Lei S Qi; Bo Huang
Journal:  Cell       Date:  2013-12-19       Impact factor: 41.582
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