Literature DB >> 33471322

TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology.

Qinlong Zhu1, Yao-Guang Liu2.   

Abstract

Efficient stacking of multiple genes is a critical element in metabolic engineering of complex pathways, synthetic biology, and genetic improvement of complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special Escherichia coli strains, and basic molecular biological means without other specific equipments.

Entities:  

Keywords:  Cre/loxP; Gene assembly; Metabolic engineering; Multigene stacking; Synthetic biology

Year:  2021        PMID： 33471322     DOI： 10.1007/978-1-0716-1068-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

Review 1.  Delivery of multiple transgenes to plant cells.

Authors:  Mery Dafny-Yelin; Tzvi Tzfira
Journal:  Plant Physiol       Date:  2007-12       Impact factor: 8.340

Review 2.  Knowledge-driven approaches for engineering complex metabolic pathways in plants.

Authors:  Gemma Farré; Richard M Twyman; Paul Christou; Teresa Capell; Changfu Zhu
Journal:  Curr Opin Biotechnol       Date:  2014-11-25       Impact factor: 9.740

Review 3.  From plant metabolic engineering to plant synthetic biology: The evolution of the design/build/test/learn cycle.

Authors:  Benjamin Pouvreau; Thomas Vanhercke; Surinder Singh
Journal:  Plant Sci       Date:  2018-04-13       Impact factor: 4.729

Review 4.  Strategies for metabolic pathway engineering with multiple transgenes.

Authors:  Ralph Bock
Journal:  Plant Mol Biol       Date:  2013-03-17       Impact factor: 4.076

5.  Development of "Purple Endosperm Rice" by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System.

Authors:  Qinlong Zhu; Suize Yu; Dongchang Zeng; Hongmei Liu; Huicong Wang; Zhongfang Yang; Xianrong Xie; Rongxin Shen; Jiantao Tan; Heying Li; Xiucai Zhao; Qunyu Zhang; Yuanling Chen; Jingxing Guo; Letian Chen; Yao-Guang Liu
Journal:  Mol Plant       Date:  2017-06-27       Impact factor: 13.164

6.  Engineering purple rice for human health.

Authors:  Xin Fang; Yingbo Mao; Xiaoya Chen
Journal:  Sci China Life Sci       Date:  2018-02-05       Impact factor: 6.038

7.  From Golden Rice to aSTARice: Bioengineering Astaxanthin Biosynthesis in Rice Endosperm.

Authors:  Qinlong Zhu; Dongchang Zeng; Suize Yu; Chaojun Cui; Jiamin Li; Heying Li; Junyu Chen; Runzhao Zhang; Xiucai Zhao; Letian Chen; Yao-Guang Liu
Journal:  Mol Plant       Date:  2018-10-06       Impact factor: 13.164

Review 8.  Engineering complex metabolic pathways in plants.

Authors:  Gemma Farré; Dieter Blancquaert; Teresa Capell; Dominique Van Der Straeten; Paul Christou; Changfu Zhu
Journal:  Annu Rev Plant Biol       Date:  2014-02-26       Impact factor: 26.379

9.  A Gateway MultiSite recombination cloning toolkit.

Authors:  Lena K Petersen; R Steven Stowers
Journal:  PLoS One       Date:  2011-09-09       Impact factor: 3.240

10.  A robust gene-stacking method utilizing yeast assembly for plant synthetic biology.

Authors:  Patrick M Shih; Khanh Vuu; Nasim Mansoori; Leïla Ayad; Katherine B Louie; Benjamin P Bowen; Trent R Northen; Dominique Loqué
Journal:  Nat Commun       Date:  2016-10-26       Impact factor: 14.919
View more
  1 in total

1.  Efficient assembly of long DNA fragments and multiple genes with improved nickase-based cloning and Cre/loxP recombination.

Authors:  Yanchang Zhao; Jingluan Han; Jiantao Tan; Yaqian Yang; Shuangchun Li; Yajun Gou; Yuyu Luo; Tie Li; Wenyu Xiao; Yang Xue; Yu Hao; Xianrong Xie; Yao-Guang Liu; Qinlong Zhu
Journal:  Plant Biotechnol J       Date:  2022-07-16       Impact factor: 13.263
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.