Felipe Barbosa de Carvalho1,2, Marcelo Gomes de Gomes3, Anne Suély Pinto Savall4, Eduarda Monteiro Fidelis4, Simone Pinton4, Ana Claudia Funguetto Ribeiro4, Félix Roman Munieweg4, Carlos Alexandre Oelke5, Sandra Elisa Haas6,7. 1. Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil. 2. Pharmacy Course, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil. 3. Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil. marcelogomesdegomes@hotmail.com. 4. Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil. 5. Department of Veterinary Medicine, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil. 6. Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil. sandrahaas@unipampa.edu.br. 7. Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil. sandrahaas@unipampa.edu.br.
Abstract
BACKGROUND: Curcumin (CUR) is a bioactive compound with several proven pharmacological properties. However, the major limitation for therapeutic use of CUR is its low bioavailability. In this sense, an alternative to this question is the use of polymeric nanocapsules (NC) as drug/nutraceutical delivery systems. Thus, the aim of current study was to assess the effect of CUR-loaded NC and their different coatings in chick embryo model, evaluating angiogenic, teratogenic and oxidative stress parameters. METHODS: The physicochemical characterization of unloaded and loaded NC with different coatings: (U-NC (P80), U-NC (PEG), U-NC (EUD), U-NC (CS), CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS)) were performed. After 9 days of incubation, eggs were treated (10 mL/kg eggs; via injection) with NC (unloaded and loaded with CUR) and CUR-solution. In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed. RESULTS: CUR-NC (P80, PEG, EUD and CS) treatments caused antiangiogenic and non-teratogenic effects in chick embryo model. Still, CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS) did not alter markers of oxidative stress (TBARS, NPSH, CAT) studied. Only CUR-NC (EUD) caused increase in ROS levels. CONCLUSION: Wherefore, these findings of present study represent a advance in research of drug/nutraceutical delivery systems.
BACKGROUND:Curcumin (CUR) is a bioactive compound with several proven pharmacological properties. However, the major limitation for therapeutic use of CUR is its low bioavailability. In this sense, an alternative to this question is the use of polymeric nanocapsules (NC) as drug/nutraceutical delivery systems. Thus, the aim of current study was to assess the effect of CUR-loaded NC and their different coatings in chick embryo model, evaluating angiogenic, teratogenic and oxidative stress parameters. METHODS: The physicochemical characterization of unloaded and loaded NC with different coatings: (U-NC (P80), U-NC (PEG), U-NC (EUD), U-NC (CS), CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS)) were performed. After 9 days of incubation, eggs were treated (10 mL/kg eggs; via injection) with NC (unloaded and loaded with CUR) and CUR-solution. In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed. RESULTS:CUR-NC (P80, PEG, EUD and CS) treatments caused antiangiogenic and non-teratogenic effects in chick embryo model. Still, CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS) did not alter markers of oxidative stress (TBARS, NPSH, CAT) studied. Only CUR-NC (EUD) caused increase in ROS levels. CONCLUSION: Wherefore, these findings of present study represent a advance in research of drug/nutraceutical delivery systems.
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