Shabnam Thakur1, Upninder Kaur1, Rakesh Sehgal2. 1. Department of Medical Parasitology, Postgraduate Institute of Medical Research and Education, Chandigarh, 160012, India. 2. Department of Medical Parasitology, Postgraduate Institute of Medical Research and Education, Chandigarh, 160012, India. sehgalpgi@gmail.com.
Abstract
OBJECTIVE: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. RESULTS: Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.
OBJECTIVE: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. RESULTS: Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.
Authors: Sitara S R Ajjampur; Premi Sankaran; Arun Kannan; Kirthi Sathyakumar; Rajiv Sarkar; Beryl P Gladstone; Gagandeep Kang Journal: Am J Trop Med Hyg Date: 2009-01 Impact factor: 2.345