Hongliang Chen1,2, Matteo Mozzicafreddo2, Elisa Pierella2, Vanessa Carletti2, Angela Piersanti2, Said M Ali3, Shaali M Ame3, Chunfeng Wang4, Cristina Miceli5. 1. College of Veterinary Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China. 2. School of Biosciences and Veterinary Medicine, University of Camerino, 62032, Camerino, Italy. 3. Public Health Laboratory Ivo de Carneri, Pemba Island, Chake Chake, Tanzania. 4. College of Veterinary Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China. wangchunfeng@jlau.edu.cn. 5. School of Biosciences and Veterinary Medicine, University of Camerino, 62032, Camerino, Italy. cristina.miceli@unicam.it.
Abstract
BACKGROUND: Soil-transmitted helminthiases are important neglected tropical diseases that result in a notably high number of disability-adjusted life years worldwide. Characterizing the interactions between the human intestinal microbiome and helminths is of interest in the development of alternative treatments that do not rely on chemotherapeutics and do not lead to drug resistance. METHODS: We recruited and obtained fecal samples from 32 pairs of mothers and children on Pemba Island and monitored their intestinal microbiota using 16S rRNA gene sequencing. RESULTS: We observed that microbial changes occur in the gut microbiota of infected mothers and children. Some short-chain fatty acid (SCFA)-producing bacteria and carbohydrate-degrading bacteria exhibited lower abundance in the infected individuals. Potentially pathogenic Campylobacter and proinflammatory Methanobrevibacter in infected mothers and opportunistic Enterococcus in infected children exhibited greater abundance. CONCLUSIONS: Our findings could reveal the microbiota profiling in T. trichiura-infected individuals, indicate the potential roles of key microbiota in the host and aid to the development of novel strategies to control T. trichiura infection.
BACKGROUND: Soil-transmitted helminthiases are important neglected tropical diseases that result in a notably high number of disability-adjusted life years worldwide. Characterizing the interactions between the human intestinal microbiome and helminths is of interest in the development of alternative treatments that do not rely on chemotherapeutics and do not lead to drug resistance. METHODS: We recruited and obtained fecal samples from 32 pairs of mothers and children on Pemba Island and monitored their intestinal microbiota using 16S rRNA gene sequencing. RESULTS: We observed that microbial changes occur in the gut microbiota of infected mothers and children. Some short-chain fatty acid (SCFA)-producing bacteria and carbohydrate-degrading bacteria exhibited lower abundance in the infected individuals. Potentially pathogenic Campylobacter and proinflammatory Methanobrevibacter in infected mothers and opportunistic Enterococcus in infectedchildren exhibited greater abundance. CONCLUSIONS: Our findings could reveal the microbiota profiling in T. trichiura-infected individuals, indicate the potential roles of key microbiota in the host and aid to the development of novel strategies to control T. trichiura infection.
Entities:
Keywords:
16S rRNA; Gut microbiota; Neglected tropical disease; Soil-transmitted helminthiases
Authors: Lisa A Reynolds; Katherine A Smith; Kara J Filbey; Yvonne Harcus; James P Hewitson; Stephen A Redpath; Yanet Valdez; María J Yebra; B Brett Finlay; Rick M Maizels Journal: Gut Microbes Date: 2014-08-05