Wei Tang1,2, Xinxing Wu1,2, Caoxing Huang1,2, Zhe Ling1,2, Chenhuan Lai1,2, Qiang Yong3,4. 1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China. 2. Key Laboratory of Forestry Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People's Republic of China. 3. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China. swhx@njfu.com.cn. 4. Key Laboratory of Forestry Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People's Republic of China. swhx@njfu.com.cn.
Abstract
BACKGROUND: Humic acids (HA) have been used in biorefinery process due to its surfactant properties as an aid to the pretreatment of lignocellulose, with results indicating a positive effect on delignification. However, the HA remaining on the surface of the pretreated lignocellulose has also been shown to provide a negative effect on ensuing enzymatic digestibility. Hence, a strategy of complexing metallic cations with HA prior to enzymatic hydrolysis was proposed and demonstrated in this work in an effort to provide a means of HA mitigation that does not involve significant water consumption via extensive washing. RESULTS: Results showed that the enzymatic hydrolysis efficiency of waste wheat straw decreased from 81.9% to 66.1% when it was pretreated by 10 g/L HA, attributed to the inhibition ability of the residual HA on enzyme activity of cellulase with a debasement of 36.3%. Interestingly, enzymatic hydrolysis efficiency could be increased from 66.1% to 77.3% when 10 mM Fe3+ was introduced to the system and allowed to associate with HA during saccharification. CONCLUSIONS: The addition of high-priced metallic cations (Fe3+) has successfully alleviated the effect of HA on cellulase activity. It is our hope in demonstrating the complexation affinity between metallic cations and HA, future researchers and biorefinery developers will evaluate this strategy as a unit operation that could allow economic biorefining of WWS to produce valuable biochemicals, biofuels, and biomaterials.
BACKGROUND:Humic acids (HA) have been used in biorefinery process due to its surfactant properties as an aid to the pretreatment of lignocellulose, with results indicating a positive effect on delignification. However, the HA remaining on the surface of the pretreated lignocellulose has also been shown to provide a negative effect on ensuing enzymatic digestibility. Hence, a strategy of complexing metallic cations with HA prior to enzymatic hydrolysis was proposed and demonstrated in this work in an effort to provide a means of HA mitigation that does not involve significant water consumption via extensive washing. RESULTS: Results showed that the enzymatic hydrolysis efficiency of waste wheat straw decreased from 81.9% to 66.1% when it was pretreated by 10 g/L HA, attributed to the inhibition ability of the residual HA on enzyme activity of cellulase with a debasement of 36.3%. Interestingly, enzymatic hydrolysis efficiency could be increased from 66.1% to 77.3% when 10 mM Fe3+ was introduced to the system and allowed to associate with HA during saccharification. CONCLUSIONS: The addition of high-priced metallic cations (Fe3+) has successfully alleviated the effect of HA on cellulase activity. It is our hope in demonstrating the complexation affinity between metallic cations and HA, future researchers and biorefinery developers will evaluate this strategy as a unit operation that could allow economic biorefining of WWS to produce valuable biochemicals, biofuels, and biomaterials.