| Literature DB >> 33466817 |
Yu Zhou1,2, Pinpin Chen1,2, Shuai Shi1,2, Xiaowen Li1,2, Deshi Shi1,2, Zutao Zhou1,2, Zili Li1,2, Yuncai Xiao1,2.
Abstract
As a multifunctional polypeptide, epidermal growth factor (EGF) increases growth performance or enhances resistance to diseases in commercial broilers under adverse conditions. In this study, a recombinant Lactococcus lactis was established to produce the secretary form of bioactive gEGF. The results of in vitro testing showed that gEGF promoted the proliferation of chicken embryo fibroblast cells. A total of 63 5-day-old broiler chickens were evenly divided into three groups and treated with either M17 medium (the control group), supernatant of LL-pNZ8149 fermentation product (the P-LL group), or supernatant of LL-pNZ8149-gEGF fermentation product (the gEGF group). In two weeks, many measurements of growth, immunity and the intestines were significantly higher in the gEGF group than those in the control and the P-LL groups. Our study showed that the bioactive gEGF could be expressed with Lactococcus lactis expression system with the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.Entities:
Keywords: Lactococcus lactis; broiler chickens; gallus epidermal growth factor; growth performance; immune function; intestinal development
Year: 2021 PMID： 33466817 PMCID： PMC7829775 DOI： 10.3390/biom11010103
Source DB: PubMed Journal: Biomolecules ISSN： 2218-273X