| Literature DB >> 33466245 |
Marie Chambard1,2, Carole Plasson1,2, Céline Derambure3, Sophie Coutant3, Isabelle Tournier3, Benjamin Lefranc4, Jérôme Leprince4, Marie-Christine Kiefer-Meyer1,2, Azeddine Driouich1,2, Marie-Laure Follet-Gueye1,2, Isabelle Boulogne1,2.
Abstract
exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a Fabaceae with worldwide interest: soybean (Glycine max (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus Phytophthora). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.Entities:
Keywords: Glycine max (L.) Merr; PEP-13; high-throughput DNA sequencing; plant exDNA; root extracellular trap (RET)
Year: 2021 PMID： 33466245 PMCID： PMC7824799 DOI： 10.3390/cells10010069
Source DB: PubMed Journal: Cells ISSN： 2073-4409 Impact factor: 6.600