Xiaojuan Xue1, Zhaorong Yu2, Hongyan Jin1,3, Lin Liang1, Jiayang Li1, Xiaolu Li1, Yong Wang1,2, Shangjin Cui1, Gang Li4. 1. Beijing Scientific Observation and Experiment Station for Veterinary Drugs and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, China /Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. 2. Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China. 3. Tibet Vocational Technical College, Lhasa, 850000, China. 4. Beijing Scientific Observation and Experiment Station for Veterinary Drugs and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, China /Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. ligang03@caas.cn.
Abstract
BACKGROUND: Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines. RESULTS: Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity. G proteins were successfully expressed with good immunogenicity. The rAds could induce the production of VSV antibodies in mice, and VSV neutralizing antibodies in goats, respectively. The neutralizing antibody titers could reach 1:32 in mice and 1:64 in goats. The rAds induced strong lymphocyte proliferation in mice and goats, which was significantly higher compared to the negative control groups. CONCLUSIONS: The three rAds constructed in the study expressed VSV-G proteins and induced both humoral and cellular immune responses in mice and goats. These results lay the foundation for further studies on the use of rAds in vaccines expressing VSV-G.
BACKGROUND:Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines. RESULTS: Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity. G proteins were successfully expressed with good immunogenicity. The rAds could induce the production of VSV antibodies in mice, and VSV neutralizing antibodies in goats, respectively. The neutralizing antibody titers could reach 1:32 in mice and 1:64 in goats. The rAds induced strong lymphocyte proliferation in mice and goats, which was significantly higher compared to the negative control groups. CONCLUSIONS: The three rAds constructed in the study expressed VSV-G proteins and induced both humoral and cellular immune responses in mice and goats. These results lay the foundation for further studies on the use of rAds in vaccines expressing VSV-G.
Authors: Andres M Perez; Steven J Pauszek; Daniel Jimenez; William N Kelley; Zachary Whedbee; Luis L Rodriguez Journal: Prev Vet Med Date: 2009-12-03 Impact factor: 2.670
Authors: Lauro Velazquez-Salinas; Steven J Pauszek; Selene Zarate; Francisco J Basurto-Alcantara; Antonio Verdugo-Rodriguez; Andres M Perez; Luis L Rodriguez Journal: Virology Date: 2013-11-14 Impact factor: 3.616