Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Calcium imaging of adult-born neurons in freely moving mice.

Literature DB >> 33458703

Calcium imaging of adult-born neurons in freely moving mice.

Alvaro Carrier-Ruiz^1,2,3, Yuki Sugaya^1,2, Deependra Kumar⁴, Pablo Vergara⁴, Iyo Koyanagi⁴, Sakthivel Srinivasan⁴, Toshie Naoi⁴, Masanobu Kano^1,2, Masanori Sakaguchi⁴.

Abstract

Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).

Entities: Chemical

Keywords: Microscopy; Model Organisms; Neuroscience

Mesh：

Year: 2020 PMID： 33458703 PMCID： PMC7797924 DOI： 10.1016/j.xpro.2020.100238

Source DB: PubMed Journal: STAR Protoc ISSN： 2666-1667

21 in total

1. In vivo analysis of Ascl1 defined progenitors reveals distinct developmental dynamics during adult neurogenesis and gliogenesis.

Authors: Euiseok J Kim; Cheuk T Leung; Randall R Reed; Jane E Johnson
Journal: J Neurosci Date: 2007-11-21 Impact factor: 6.167

2. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.

Authors: Shanna L Resendez; Josh H Jennings; Randall L Ung; Vijay Mohan K Namboodiri; Zhe Charles Zhou; James M Otis; Hiroshi Nomura; Jenna A McHenry; Oksana Kosyk; Garret D Stuber
Journal: Nat Protoc Date: 2016-02-25 Impact factor: 13.491

3. Active Dentate Granule Cells Encode Experience to Promote the Addition of Adult-Born Hippocampal Neurons.

Authors: Gregory W Kirschen; Jia Shen; Mu Tian; Bryce Schroeder; Jia Wang; Guoming Man; Song Wu; Shaoyu Ge
Journal: J Neurosci Date: 2017-04-03 Impact factor: 6.167

4. Causal evidence for the role of REM sleep theta rhythm in contextual memory consolidation.

Authors: Richard Boyce; Stephen D Glasgow; Sylvain Williams; Antoine Adamantidis
Journal: Science Date: 2016-05-13 Impact factor: 47.728

5. Parallel emergence of stable and dynamic memory engrams in the hippocampus.

Authors: Thomas Hainmueller; Marlene Bartos
Journal: Nature Date: 2018-06-06 Impact factor: 49.962

6. Polygraphic Recording Procedure for Measuring Sleep in Mice.

Authors: Yo Oishi; Yohko Takata; Yujiro Taguchi; Sayaka Kohtoh; Yoshihiro Urade; Michael Lazarus
Journal: J Vis Exp Date: 2016-01-25 Impact factor: 1.355

7. Tracking the Same Neurons across Multiple Days in Ca²⁺ Imaging Data.

Authors: Liron Sheintuch; Alon Rubin; Noa Brande-Eilat; Nitzan Geva; Noa Sadeh; Or Pinchasof; Yaniv Ziv
Journal: Cell Rep Date: 2017-10-24 Impact factor: 9.423

8. Orchestrated ensemble activities constitute a hippocampal memory engram.

Authors: Khaled Ghandour; Noriaki Ohkawa; Chi Chung Alan Fung; Hirotaka Asai; Yoshito Saitoh; Takashi Takekawa; Reiko Okubo-Suzuki; Shingo Soya; Hirofumi Nishizono; Mina Matsuo; Makoto Osanai; Masaaki Sato; Masamichi Ohkura; Junichi Nakai; Yasunori Hayashi; Takeshi Sakurai; Takashi Kitamura; Tomoki Fukai; Kaoru Inokuchi
Journal: Nat Commun Date: 2019-06-14 Impact factor: 14.919

Calcium imaging of adult-born neurons in freely moving mice.

1. In vivo analysis of Ascl1 defined progenitors reveals distinct developmental dynamics during adult neurogenesis and gliogenesis.

2. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.

3. Active Dentate Granule Cells Encode Experience to Promote the Addition of Adult-Born Hippocampal Neurons.

4. Causal evidence for the role of REM sleep theta rhythm in contextual memory consolidation.

5. Parallel emergence of stable and dynamic memory engrams in the hippocampus.

6. Polygraphic Recording Procedure for Measuring Sleep in Mice.

7. Tracking the Same Neurons across Multiple Days in Ca²⁺ Imaging Data.

8. Orchestrated ensemble activities constitute a hippocampal memory engram.

9. Reliable Genetic Labeling of Adult-Born Dentate Granule Cells Using Ascl1 CreERT2 and Glast CreERT2 Murine Lines.

10. Sleep: An Open-Source Python Software for Visualization, Analysis, and Staging of Sleep Data.

Review 1. Adult hippocampal neurogenesis and its impairment in Alzheimer's disease.

2. Two photon imaging of calcium responses in murine Purkinje neurons.

Review 3. Adult Neurogenesis under Control of the Circadian System.

4. Remapping of Adult-Born Neuron Activity during Fear Memory Consolidation in Mice.

5. Miniature microscopes for manipulating and recording in vivo brain activity.