Literature DB >> 3345842

Interaction of site specific hirudin variants with alpha-thrombin.

J Dodt1, S Köhler, A Baici.   

Abstract

The kinetics of complex formation between recombinant hirudin or recombinant hirudin mutants with thrombin were analyzed. In order to elucidate the inhibitor's reactive site peptide bond predetermined amino acid substitutions were introduced at positions of basic amino acid residues by means of site-directed mutagenesis of a hirudin gene. In comparison to recombinant hirudin (Ki = 19 pM) only those mutant inhibitors which were modified at amino acid position Lys47 showed a higher Ki value for their complexes with thrombin. The observed effects are mainly due to increased koff rate constants.

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Year:  1988        PMID： 3345842     DOI： 10.1016/0014-5793(88)80803-2

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  11 in total

1.  Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships.

Authors:  Christian Müller; Martin Haase; Sarah Lemke; Jan-Peter Hildebrandt
Journal:  Parasitol Res       Date:  2016-10-27       Impact factor: 2.289

2.  Hirudins of the Asian medicinal leech, Hirudinaria manillensis: same same, but different.

Authors:  Phil Lukas; Robert Wolf; Bernhard H Rauch; Jan-Peter Hildebrandt; Christian Müller
Journal:  Parasitol Res       Date:  2019-06-11       Impact factor: 2.289

3.  Changes in interactions in complexes of hirudin derivatives and human alpha-thrombin due to different crystal forms.

Authors:  J P Priestle; J Rahuel; H Rink; M Tones; M G Grütter
Journal:  Protein Sci       Date:  1993-10       Impact factor: 6.725

4.  The refined 1.9-A X-ray crystal structure of D-Phe-Pro-Arg chloromethylketone-inhibited human alpha-thrombin: structure analysis, overall structure, electrostatic properties, detailed active-site geometry, and structure-function relationships.

Authors:  W Bode; D Turk; A Karshikov
Journal:  Protein Sci       Date:  1992-04       Impact factor: 6.725

5.  More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis.

Authors:  Christian Müller; Katharina Mescke; Stephanie Liebig; Hala Mahfoud; Sarah Lemke; Jan-Peter Hildebrandt
Journal:  Mol Genet Genomics       Date:  2015-08-13       Impact factor: 3.291

6.  Thrombin-specific inhibition by and slow cleavage of hirulog-1.

Authors:  J I Witting; P Bourdon; D V Brezniak; J M Maraganore; J W Fenton
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

7.  Crystal structure of thrombin in complex with S-variegin: insights of a novel mechanism of inhibition and design of tunable thrombin inhibitors.

Authors:  Cho Yeow Koh; Sundramurthy Kumar; Maria Kazimirova; Patricia A Nuttall; Uvaraj P Radhakrishnan; Seongcheol Kim; Pudur Jagadeeswaran; Takayuki Imamura; Jun Mizuguchi; Sadaaki Iwanaga; Kunchithapadam Swaminathan; R Manjunatha Kini
Journal:  PLoS One       Date:  2011-10-28       Impact factor: 3.240

8.  Revisiting antithrombotic therapeutics; sculptin, a novel specific, competitive, reversible, scissile and tight binding inhibitor of thrombin.

Authors:  Asif Iqbal; Mauricio Barbugiani Goldfeder; Rafael Marques-Porto; Huma Asif; Jean Gabriel de Souza; Fernanda Faria; Ana Marisa Chudzinski-Tavassi
Journal:  Sci Rep       Date:  2017-05-03       Impact factor: 4.379

9.  Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.

Authors:  M G Grütter; J P Priestle; J Rahuel; H Grossenbacher; W Bode; J Hofsteenge; S R Stone
Journal:  EMBO J       Date:  1990-08       Impact factor: 11.598

10.  Two heads are better than one: crystal structure of the insect derived double domain Kazal inhibitor rhodniin in complex with thrombin.

Authors:  A van de Locht; D Lamba; M Bauer; R Huber; T Friedrich; B Kröger; W Höffken; W Bode
Journal:  EMBO J       Date:  1995-11-01       Impact factor: 11.598
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