Literature DB >> 3345838

Do DNA polymerases delta and alpha act coordinately as leading and lagging strand replicases?

F Focher1, E Ferrari, S Spadari, U Hübscher.   

Abstract

The activity ratio of DNA polymerases delta and alpha in calf thymus was found to be invariably 1:1, irrespective of extraction procedure (8 types) and subcellular localization (cytoplasm, nucleus and microsomes). This was established by separation of the two forms by hydroxyapatite chromatography and by their response to specific inhibitors and monoclonal antibodies. This finding supports the dimeric DNA polymerase model [(1980) J. Biol. Chem. 255, 4290-4303], which proposes that DNA polymerases delta and alpha act coordinately as leading and lagging strand enzymes, respectively, at the replication fork.

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Year:  1988        PMID： 3345838     DOI： 10.1016/0014-5793(88)80786-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Exonucleolytic proofreading of leading and lagging strand DNA replication errors.

Authors:  J D Roberts; D C Thomas; T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

2.  Aphidicolin-resistant human cell lines resistant to UV and bleomycin.

Authors:  K Kamino; K Fukuchi; K Tanaka; T Miki; J Nakura; Y Takemoto; Y Kumahara; T Ogihara
Journal:  In Vitro Cell Dev Biol       Date:  1993-03

3.  Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40.

Authors:  T Nethanel; G Kaufmann
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

4.  Contribution of DNA polymerase delta to DNA replication in permeable CHO cells synchronized in S phase.

Authors:  A Basnakian; G Banfalvi; N Sarkar
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

5.  Structure-activity relationships for the inhibition of DNA polymerase alpha by aphidicolin derivatives.

Authors:  G Prasad; R A Edelson; P D Gorycki; T L Macdonald
Journal:  Nucleic Acids Res       Date:  1989-08-11       Impact factor: 16.971

6.  Calf thymus DNA polymerase delta independent of proliferating cell nuclear antigen (PCNA).

Authors:  F Focher; M Gassmann; P Hafkemeyer; E Ferrari; S Spadari; U Hübscher
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

7.  The molecular mechanism of inhibition of alpha-type DNA polymerases by N2-(butylphenyl)dGTP and 2-(butylanilino)dATP: variation in susceptibility to polymerization.

Authors:  N N Khan; G E Wright; N C Brown
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

8.  Calf thymus DNA polymerase delta: purification, biochemical and functional properties of the enzyme after its separation from DNA polymerase alpha, a DNA dependent ATPase and proliferating cell nuclear antigen.

Authors:  F Focher; S Spadari; B Ginelli; M Hottiger; M Gassmann; U Hübscher
Journal:  Nucleic Acids Res       Date:  1988-07-25       Impact factor: 16.971

9.  Proliferating cell nuclear antigen/cyclin in the ciliate Euplotes eurystomus: localization in the replication band and in micronuclei.

Authors:  D E Olins; A L Olins; L H Cacheiro; E M Tan
Journal:  J Cell Biol       Date:  1989-10       Impact factor: 10.539
  9 in total

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