| Literature DB >> 3345820 |
T Erb1, G Karolyi, A Pampalone, A N Eberle, J B Baumann, E Bürgisser, J Girard.
Abstract
An immunoradiometric assay for human growth hormone (HGH) has been developed which has a detection limit of 1 ng/l and can measure HGH in unextracted urine from normal children and adults. The assay is based on a two-step procedure, using a solid-phase goat-anti-HGH immunosorbent for immunoextraction and [125I]-labeled monoclonal HGH-antibody for detection and quantification. The assay is not affected by urea, NaCl or changes of pH from 5-8. The mean urine HGH concentration in normal children is 6.78 +/- 7.6 (SD) pg/ml, in patients with HGH-deficiency 1.3 +/- 0.9 pg/ml which increases to 11.7 +/- 13.4 pg/ml on the day of growth hormone injection.Entities:
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Year: 1988 PMID： 3345820 DOI： 10.1007/bf01952201
Source DB: PubMed Journal: Experientia ISSN： 0014-4754