Jinshi Zhang1,2,3,4, Yifan Zhu2,3,4, Ruyi Cai1,2,3,4, Juan Jin2,3,4, Qiang He2,3,4. 1. School of Medicine, Hangzhou Normal University, Zhejiang 310018, China. 2. Department of Nephrology, Zhejiang Provincial People's Hospital, Zhejiang 310014, China. 3. People's Hospital of Hangzhou Medical College, Zhejiang 310014, China. 4. Chinese Medical Nephrology Key Laboratory of Zhejiang Province, Hangzhou, Zhejiang 310014, China.
Abstract
BACKGROUND: Idiopathic membranous nephropathy (IMN) is a major cause of adult nephrotic syndromes, and reliable noninvasive biomarkers for diagnosis and monitoring are urgently needed. In this study, we performed small RNA (sRNA) sequencing to explore sRNA profiles of urinary exosomes derived from IMN patients and healthy controls (CON) to provide clues for identifying novel noninvasive sRNA biomarkers for IMN. METHODS: Urine samples were collected from five healthy controls and six patients with IMN. High-throughput sequencing was used to screen sRNA expression profiles of urinary exosomes from patients with IMN in two independent cohorts. RESULTS: Urinary exosomes were successfully isolated and used to obtain exosomal sRNAs. We screened 131 differentially expressed miRNAs, including 28 specifically expressed miRNAs, then explored the top 10 specifically expressed miRNAs in all IMN individuals. The specifically expressed miRNAs and differentially expressed miRNAs provide potential biomarkers for IMN. Additionally, we discovered numerous sRNAs derived from genomic repetitive sequences, which could represent an exciting new area of research. CONCLUSION: Herein, we revealed significant differences in expression profiles of urinary exosomal miRNAs and repetitive region-derived sRNAs between patients with IMN and healthy controls. The findings could facilitate the development of potential molecular targets for membranous nephropathy.
BACKGROUND: Idiopathic membranous nephropathy (IMN) is a major cause of adult nephrotic syndromes, and reliable noninvasive biomarkers for diagnosis and monitoring are urgently needed. In this study, we performed small RNA (sRNA) sequencing to explore sRNA profiles of urinary exosomes derived from IMN patients and healthy controls (CON) to provide clues for identifying novel noninvasive sRNA biomarkers for IMN. METHODS: Urine samples were collected from five healthy controls and six patients with IMN. High-throughput sequencing was used to screen sRNA expression profiles of urinary exosomes from patients with IMN in two independent cohorts. RESULTS: Urinary exosomes were successfully isolated and used to obtain exosomal sRNAs. We screened 131 differentially expressed miRNAs, including 28 specifically expressed miRNAs, then explored the top 10 specifically expressed miRNAs in all IMN individuals. The specifically expressed miRNAs and differentially expressed miRNAs provide potential biomarkers for IMN. Additionally, we discovered numerous sRNAs derived from genomic repetitive sequences, which could represent an exciting new area of research. CONCLUSION: Herein, we revealed significant differences in expression profiles of urinary exosomal miRNAs and repetitive region-derived sRNAs between patients with IMN and healthy controls. The findings could facilitate the development of potential molecular targets for membranous nephropathy.
Authors: Javier Perez-Hernandez; Dolores Olivares; Maria J Forner; Ana Ortega; Elena Solaz; Fernando Martinez; Felipe J Chaves; Josep Redon; Raquel Cortes Journal: J Transl Med Date: 2018-08-14 Impact factor: 5.531
Authors: Daniel S K Liu; Qi Zhi Clayton Yang; Mohammad Asim; Jonathan Krell; Adam E Frampton Journal: Int J Mol Sci Date: 2022-03-28 Impact factor: 5.923