Igor Sergeevich Brodetskyi1, Vladislav O Malanchuk1, Victor E Dosenko2. 1. Department of Oral and Maxillofacial Surgery, Bogomolets National Medical University, Kyiv, Ukraine. 2. Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine.
Abstract
BACKGROUND: The most common type of salivary gland tumor is pleomorphic adenoma. The genetic area of focus in the diagnosis of salivary gland tumors is a study of the role of miRNA. METHODS: Twenty-two patients with pleomorphic adenomas of the salivary glands were used for the examinations. The histological typing of the salivary gland tumors was performed when using routine staining with hematoxylin and eosin, as well as with immunohistochemistry. The expressions of miR-34a and miR-29a were evaluated by using reverse transcription and the quantitative polymerase chain reaction in a real-time setting. In addition, the study also calculated the levels of expression of miR-29a and miR-34a in the venous blood. RESULTS: The majority of patients-15 (68.18%) and 22 (100.00%) had a positive response to human papillomavirus (HPV) and pleomorphic adenoma gene 1 (PLAG1), respectively. The conducted analyses of the expressions of miR-34a and miR-29a showed that the highest expression was observed in the salivary gland tissue adjacent to the tumor (1,052.02±367.20 and 111.93±56.97, versus 47.72±28.93 and 8.12±4.40 in the intact salivary gland tissue, respectively). CONCLUSIONS: There was a sufficiently high level of miR-34a and miR-29a expressions in the tissues of the tumor of pleomorphic adenomas of the salivary glands when compared with the intact salivary gland tissue. 2020 Gland Surgery. All rights reserved.
BACKGROUND: The most common type of salivary gland tumor is pleomorphic adenoma. The genetic area of focus in the diagnosis of salivary gland tumors is a study of the role of miRNA. METHODS: Twenty-two patients with pleomorphic adenomas of the salivary glands were used for the examinations. The histological typing of the salivary gland tumors was performed when using routine staining with hematoxylin and eosin, as well as with immunohistochemistry. The expressions of miR-34a and miR-29a were evaluated by using reverse transcription and the quantitative polymerase chain reaction in a real-time setting. In addition, the study also calculated the levels of expression of miR-29a and miR-34a in the venous blood. RESULTS: The majority of patients-15 (68.18%) and 22 (100.00%) had a positive response to human papillomavirus (HPV) and pleomorphic adenoma gene 1 (PLAG1), respectively. The conducted analyses of the expressions of miR-34a and miR-29a showed that the highest expression was observed in the salivary gland tissue adjacent to the tumor (1,052.02±367.20 and 111.93±56.97, versus 47.72±28.93 and 8.12±4.40 in the intact salivary gland tissue, respectively). CONCLUSIONS: There was a sufficiently high level of miR-34a and miR-29a expressions in the tissues of the tumor of pleomorphic adenomas of the salivary glands when compared with the intact salivary gland tissue. 2020 Gland Surgery. All rights reserved.
Authors: Yulian Mytsyk; Victor Dosenko; Yuriy Borys; Askold Kucher; Katarina Gazdikova; Dietrich Busselberg; Martin Caprnda; Peter Kruzliak; Ammad Ahmad Farooqi; Manyuk Lubov Journal: Int Urol Nephrol Date: 2018-03-16 Impact factor: 2.370
Authors: Maria Denaro; Elena Navari; Clara Ugolini; Veronica Seccia; Valentina Donati; Augusto Pietro Casani; Fulvio Basolo Journal: PLoS One Date: 2019-01-25 Impact factor: 3.240