Characterization of sucrose non-fermenting-1 (SNF1) homologue gene in Fusarium udum WSP-V2 and its regulation by the biocontrol agent Pseudomonas fluorescens OKC.

Sucrose non-fermenting 1 (SNF1) is a protein kinase and plays an important role in the energy homeostasis of glucose repressible gene transcription. It derepresses glucose repressed genes and associated with pathogenesis and production of cell wall degrading enzymes in fungal species. In the present study, we identified and characterized SNF1 homologue FuSNF1 in the F. udum strain WSP-V2. Transcript analysis of FuSNF1 along with the MAP kinases and some cell wall degrading enzyme (CWDE) genes of F. udum during interaction with pigeonpea revealed that most MAP kinases and CWDE genes was positively correlated with the FuSNF1 gene. Interestingly, transcript accumulation of all these genes was lowered when pigeonpea seeds were bioprimed with a PGPR strain Pseudomonas fluorescens OKC. Transcript accumulation of FuSNF1 was observed from the day of inoculation and reached maximum level on day 7 in OKC non-bioprimed plants. However, transcript accumulation was low (1.5 fold) in F. udum inoculated with pigeonpea plants bioprimed with OKC. Transcript accumulation patterns of the F. udum MAP Kinases genes and CWDE genes also showed a similar trend and their transcript accumulation was lowered in the OKC bioprimed treatment. The results thus indicate a prime role of FuSNF1 in regulating pathogenicity and virulence of F. udum. The results further emphasize the importance of application of effective PGPR strains in regulating virulence of F. udum. In silico analysis of the SNF1 reference proteins from different fungal species showed that their homologue FuSNF1 is likely to be thermostable and acidic in nature. © King Abdulaziz City for Science and Technology 2021.