| Literature DB >> 33441565 |
Shuai Zhao1,2,3, Run Tian1,2, Jun Wu4, Shaoli Liu1,2, Yuanning Wang1, Meng Wen4, Yingxu Shang1,2, Qing Liu1, Yan Li1, Ying Guo5, Zhaoran Wang1,2, Ting Wang1, Yujing Zhao4, Huiru Zhao4, Hui Cao4, Yu Su4, Jiashu Sun1,2, Qiao Jiang6,7, Baoquan Ding8,9,10.
Abstract
Effective and safe hemodialysis is essential for patients with acute kidney injury and chronic renal failures. However, the development of effective anticoagulant agents with safe antidotes for use during hemodialysis has proven challenging. Here, we describe DNA origami-based assemblies that enable the inhibition of thrombin activity and thrombus formation. Two different thrombin-binding aptamers decorated DNA origami initiates protein recognition and inhibition, exhibiting enhanced anticoagulation in human plasma, fresh whole blood and a murine model. In a dialyzer-containing extracorporeal circuit that mimicked clinical hemodialysis, the origami-based aptamer nanoarray effectively prevented thrombosis formation. Oligonucleotides containing sequences complementary to the thrombin-binding aptamers can efficiently neutralize the anticoagulant effects. The nanoarray is safe and immunologically inert in healthy mice, eliciting no detectable changes in liver and kidney functions or serum cytokine concentration. This DNA origami-based nanoagent represents a promising anticoagulant platform for the hemodialysis treatment of renal diseases.Entities:
Year: 2021 PMID： 33441565 DOI： 10.1038/s41467-020-20638-7
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919