Mona Saheli1, Vahid Pirhajati Mahabadi2,3, Seyed Alireza Mesbah-Namin4, Alexander Seifalian5, Zahra Bagheri-Hosseinabadi6,7. 1. Department of Anatomical Sciences, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran. 2. Neuroscience Research Center, Vice-Chancellor for Research and Technology, Iran University of Medical Sciences, Tehran, Iran. 3. Cellular and Molecular Research Center, Vice-Chancellor for Research and Technology, Iran university of Medical Sciences, Tehran, Iran. 4. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 5. Nanotechnology and Regenerative Medicine Commercialisation Centre (NanoRegMed Ltd.), London BioScience Innovation Centre, London, UK. 6. Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. 7. Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Abstract
BACKGROUND: The adult human heart muscle cells, cardiomyocytes are not capable of regenerate after injury. Stem cells are a powerful means for future regenerative medicine because of their capacity for self-renewal and multipotency. Several studies have reported the cardiogenic potential in human adipose tissue-derived stem cells (ADSCs) differentiation, but there is still no efficient protocol for the induction of cardiac differentiation by 5-azacytidine (5-Aza). The present study involves characterization and mainly, the ultrastructure of ADSCs derived cardiomyocyte-like cells. METHODS: The cultured ADSCs were treated with 50 µM 5-Aza for 24 hours, followed by a 10-week extension. At different time points, cardiomyocyte-like cells were assessed by qRT-PCR and were evaluated by transmission electron microscopy at 10th week. RESULTS: The expression of cardiac-specific markers entailing cardiac troponin I (cTnI), connexin 43, myosin light chain-2v (Mlc-2v), increased over 10 weeks and the highest expression was at 10th week. The expression of the β-myosin heavy chain (β-MHC) increased significantly over 5 weeks and then decreased. At the ultrastructural level myofibrils, transverse tubules (T-tubules), sarcoplasmic reticular membrane, and intercalated discs were present. CONCLUSIONS: These data suggest that treatment with 5-Aza in high dose could promote differentiation of ADSCs into cardiomyocyte-like cells. These differentiated cells could be used for regeneration of damaged cardiomyocytes with the 3D scaffold for delivery of the cells. 2020 Stem Cell Investigation. All rights reserved.
BACKGROUND: The adult human heart muscle cells, cardiomyocytes are not capable of regenerate after injury. Stem cells are a powerful means for future regenerative medicine because of their capacity for self-renewal and multipotency. Several studies have reported the cardiogenic potential in human adipose tissue-derived stem cells (ADSCs) differentiation, but there is still no efficient protocol for the induction of cardiac differentiation by 5-azacytidine (5-Aza). The present study involves characterization and mainly, the ultrastructure of ADSCs derived cardiomyocyte-like cells. METHODS: The cultured ADSCs were treated with 50 µM 5-Aza for 24 hours, followed by a 10-week extension. At different time points, cardiomyocyte-like cells were assessed by qRT-PCR and were evaluated by transmission electron microscopy at 10th week. RESULTS: The expression of cardiac-specific markers entailing cardiac troponin I (cTnI), connexin 43, myosin light chain-2v (Mlc-2v), increased over 10 weeks and the highest expression was at 10th week. The expression of the β-myosin heavy chain (β-MHC) increased significantly over 5 weeks and then decreased. At the ultrastructural level myofibrils, transverse tubules (T-tubules), sarcoplasmic reticular membrane, and intercalated discs were present. CONCLUSIONS: These data suggest that treatment with 5-Aza in high dose could promote differentiation of ADSCs into cardiomyocyte-like cells. These differentiated cells could be used for regeneration of damaged cardiomyocytes with the 3D scaffold for delivery of the cells. 2020 Stem Cell Investigation. All rights reserved.
Authors: Marius Strioga; Sowmya Viswanathan; Adas Darinskas; Ondrej Slaby; Jaroslav Michalek Journal: Stem Cells Dev Date: 2012-05-09 Impact factor: 3.272
Authors: Lucie Bacakova; Jana Zarubova; Martina Travnickova; Jana Musilkova; Julia Pajorova; Petr Slepicka; Nikola Slepickova Kasalkova; Vaclav Svorcik; Zdenka Kolska; Hooman Motarjemi; Martin Molitor Journal: Biotechnol Adv Date: 2018-03-18 Impact factor: 14.227
Authors: Sander F Rodrigo; Jan van Ramshorst; Georgette E Hoogslag; Helèn Boden; Matthijs A Velders; Suzanne C Cannegieter; Helene Roelofs; Imad Al Younis; Petra Dibbets-Schneider; Willem E Fibbe; Jaap Jan Zwaginga; Jeroen J Bax; Martin J Schalij; Saskia L Beeres; Douwe E Atsma Journal: J Cardiovasc Transl Res Date: 2013-08-28 Impact factor: 4.132